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Literature summary extracted from

  • Renosto, F.; Schultz, T.; Re, E.; Mazer, J.; Chandler, C.J.; Barron, A.; Segel, I.H.
    Comparative stability and catalytic and chemical properties of the sulfate-activating enzymes from Penicillium chrysogenum (mesophile) and Penicillium duponti (thermophile) (1985), J. Bacteriol., 164, 674-683.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 FSO3-
-
 Penicillium chrysogenum
2.7.7.4 FSO3-
-
 Penicillium duponti
2.7.7.4 S2O32-
-
 Penicillium chrysogenum
2.7.7.4 S2O32-
-
 Penicillium duponti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.0003
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.0003
-
 adenosine 5'-phosphosulfate pH 8.0, 30°C Penicillium duponti
2.7.7.4 0.0065
-
 diphosphate pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.0083
-
 diphosphate pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.03
-
 ATP pH 8.0, 30°C, at saturating concentrations of MoO42- Penicillium duponti
2.7.7.4 0.03
-
 ATP ATP in form of MgATP2- Penicillium duponti
2.7.7.4 0.05
-
 ATP pH 8.0, 30°C, at saturating concentrations of MoO42- Penicillium chrysogenum
2.7.7.4 0.05
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum
2.7.7.4 0.08
-
 MoO42- pH 8.0, 30°C Penicillium duponti
2.7.7.4 0.11
-
 MoO42- pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.18
-
 ATP pH 8.0, 30°C, at saturating concentrations of SO42- Penicillium chrysogenum
2.7.7.4 0.18
-
 ATP ATP in form of MgATP2- Penicillium chrysogenum
2.7.7.4 0.19
-
 ATP pH 8.0, 30°C, at saturating concentrations of SO42- Penicillium duponti
2.7.7.4 0.19
-
 ATP ATP in form of MgATP2- Penicillium duponti
2.7.7.4 0.55
-
 SO42- pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.55
-
 SO42- pH 8.0, 30°C Penicillium duponti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ activation Penicillium chrysogenum
2.7.1.25 Mg2+ activation Penicillium duponti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 33000
-
 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum
2.7.1.25 57000
-
 gel filtration at 22°C Penicillium chrysogenum
2.7.1.25 57000
-
 gel filtration at 22°C or 46°C Penicillium duponti
2.7.7.4 69000
-
 6 * 69000, SDS-PAGE Penicillium chrysogenum
2.7.7.4 440000
-
 gel filtration Penicillium chrysogenum
2.7.7.4 440000
-
 gel filtration Penicillium duponti

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Penicillium chrysogenum
-
-
-
2.7.1.25 Penicillium duponti
-
-
-
2.7.7.4 Penicillium chrysogenum
-
-
-
2.7.7.4 Penicillium duponti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25
-
 Penicillium chrysogenum
2.7.1.25 partial Penicillium duponti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.25 mycelium
-
 Penicillium chrysogenum
-
2.7.1.25 mycelium
-
 Penicillium duponti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium duponti ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate r
2.7.7.4 ATP + molybdate
-
 Penicillium chrysogenum diphosphate + adenylyl molybdate
-
 r
2.7.7.4 ATP + molybdate
-
 Penicillium duponti diphosphate + adenylyl molybdate
-
 r
2.7.7.4 ATP + MoO42-
-
 Penicillium chrysogenum AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + MoO42-
-
 Penicillium duponti AMP + adenylylmolybdate
-
 ?
2.7.7.4 ATP + sulfate
-
 Penicillium chrysogenum diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Penicillium duponti diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Penicillium chrysogenum diphosphate + adenylyl sulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Penicillium duponti diphosphate + adenylyl sulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 33000, gel filtration at 46°C, the enzyme dissociates into two inactive monomers by heating above 42°C Penicillium chrysogenum
2.7.7.4 hexamer 6 * 69000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.4 20
-
 in a 20 min assay Penicillium chrysogenum
2.7.7.4 20
-
 in a 20 min assay Penicillium duponti

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.25 35
-
 inactivation above, reversible by cooling to 0-30°C Penicillium chrysogenum
2.7.7.4 65
-
 enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme Penicillium chrysogenum
2.7.7.4 65
-
 enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme Penicillium duponti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.4 4.4
-
 enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme Penicillium chrysogenum
2.7.7.4 4.4
-
 enzyme of Penicillium chrysogenum denaturates with a rate constant nearly 100fold greater than that of the Penicillium duponti enzyme Penicillium duponti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.4 0.0034
-
 FSO3- pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.004
-
 FSO3- pH 8.0, 30°C Penicillium duponti
2.7.7.4 0.36
-
 S2O32- pH 8.0, 30°C Penicillium chrysogenum
2.7.7.4 0.4
-
 S2O32- pH 8.0, 30°C Penicillium duponti