EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | additional information | not inhibited by adenosine 5'-phosphosulfate | Geobacillus stearothermophilus | |
2.7.1.25 | additional information | - |
Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.25 | 0.042 | - |
adenosine 5'-phosphosulfate | pH 8.0, 30°C | Geobacillus stearothermophilus | |
2.7.1.25 | 0.22 | - |
ATP | pH 8.0, 30°C | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.25 | Co2+ | 164% of activation with Mg2+ | Geobacillus stearothermophilus | |
2.7.1.25 | Mg2+ | - |
Geobacillus stearothermophilus | |
2.7.1.25 | Mn2+ | 274% of activation with Mg2+ | Geobacillus stearothermophilus | |
2.7.1.25 | Zn2+ | 309% of activation with Mg2+ | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 26000 | - |
2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
2.7.1.25 | 50000 | - |
gel filtration | Geobacillus stearothermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.25 | Geobacillus stearothermophilus | - |
- |
- |
2.7.1.25 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.25 | DEAE-Sepharose, Matrex gel blue A, phenyl Cellofine | Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 1.7 | - |
- |
Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Saccharomyces cerevisiae | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? | |
2.7.1.25 | ATP + adenosine 5-phosphosulfate | i.e. adenylylsulfate or APS | Geobacillus stearothermophilus | ADP + 3'-phosphoadenosine 5'-phosphosulfate | i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.25 | dimer | - |
Saccharomyces cerevisiae |
2.7.1.25 | dimer | 2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 25 | - |
no loss of activity after 7 d at room temperatures | Geobacillus stearothermophilus |
2.7.1.25 | 25 | - |
70% loss of activity after 7 d at room temperature | Saccharomyces cerevisiae |
2.7.1.25 | 60 | - |
complete inactivation after 15 min | Saccharomyces cerevisiae |
2.7.1.25 | 70 | - |
no significant loss of activity after 15 min | Geobacillus stearothermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.25 | 6.8 | 7 | - |
Saccharomyces cerevisiae |
2.7.1.25 | 7 | 8 | - |
Geobacillus stearothermophilus |