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Literature summary extracted from

  • Satishchandran, C.; Markham, G.D.
    Mechanistic studies of Escherichia coli adenosine-5'-phosphosulfate kinase (2000), Arch. Biochem. Biophys., 378, 210-215.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.0005
-
 adenosine 5'-phosphosulfate pH 8.0 Escherichia coli
2.7.1.25 0.06
-
 adenosine 5'-O-(3-thiotriphosphate) pH 8.0 Escherichia coli
2.7.1.25 0.13
-
 ADP ADP in form of MgADP- Escherichia coli
2.7.1.25 0.37
-
 3'-phosphoadenosine 5'-phosphosulfate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 Mg2+ affinity for adenosine 5'-phosphosulfate decreases by 15fold in the absence of Mg2+ Escherichia coli
2.7.1.25 Mn2+ 1 Mn2+ ion per subunit in the absence of substrate, 2 Mn2+ bind per subunit when APS or the ATP analog 5'-adenylylimidodiphosphate are present, afffinity for Mn2+ increases 23fold when the enzyme is phosphorylated Escherichia coli
2.7.1.25 additional information enzyme uses multiple metal ions to catalyze phosphoryl group transfer Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.25 ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate kinetic scheme for APS kinase Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 adenosine 5'-O-(3-thiotriphosphate) + adenosine 5-phosphosulfate 535fold lower Vmax than with ATP Escherichia coli ADP + 3'-thiophosphoadenosine 5'-phosphosulfate
-
 ?
2.7.1.25 ADP + 3'-phosphoadenylyl sulfate
-
 Escherichia coli ATP + adenylyl sulfate
-
 r
2.7.1.25 ATP + adenosine 5'-O-(2-fluorodiphosphate) 15fold lower Vmax than with adenosine 5-phosphosulfate Escherichia coli ADP + 3'-phosphoadenosine 5'-O-(2-fluorodiphosphate)
-
 ?
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Escherichia coli ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.25 0.09
-
 adenosine 5'-O-(3-thiotriphosphate) pH 8.0 Escherichia coli