Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lillig, C.H.; Schiffmann, S.; Berndt, C.; Berken, A.; Tischka, R.; Schwenn, J.D.
    Molecular and catalytic properties of Arabidopsis thaliana adenylyl sulfate (APS)-kinase (2001), Arch. Biochem. Biophys., 392, 303-310.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.25 dithiothreitol activity declines rapidly in the absence of dithiothreitol Arabidopsis thaliana
2.7.1.25 high ionic strength maximal activity at a high concentration of buffer-salts either alone or plus dithiothreitol and thioredoxin 1 from Escherichia coli Arabidopsis thaliana
2.7.1.25 thioredoxin essential for activity Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.25 S182C similar catalytic activity as wild-type Arabidopsis thaliana
2.7.1.25 S182F similar catalytic activity as wild-type Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.7.1.25 recombinant enzyme is rapidly inactivated by dialysis, dilution or freezing even in the presence of glycerol Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate uncompetitive inhibition above 0.001 mM Arabidopsis thaliana
2.7.1.25 EDTA complete inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.25 0.00014
-
 adenosine 5'-phosphosulfate pH 7.4, 25°C Arabidopsis thaliana
2.7.1.25 0.147
-
 ATP pH 7.4, 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.25 chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 31977
-
 x * 31977, deduced from nucleotide sequence Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Arabidopsis thaliana O49196 APS kinase gene Atakn1
-
2.7.1.25 Arabidopsis thaliana O49196 APS kinase gene Atakn2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 ? x * 31977, deduced from nucleotide sequence Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.25 0.0045
-
 adenosine 5'-phosphosulfate pH 7.4, 25°C Arabidopsis thaliana