Literature summary extracted from
Lansdon, E.B.; Segel, I.H.; Fisher, A.J.
Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum (2002), Biochemistry, 41, 13672-13680.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.1.25 |
crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A |
Penicillium chrysogenum |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.1.25 |
adenosine 5'-phosphosulfate |
adenosine 5'-phosphosulfate can bind to E-MgADP forming a catalytically inactive E-MgADP-APS ternary complex |
Penicillium chrysogenum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.1.25 |
23670 |
- |
2 * 23670 |
Penicillium chrysogenum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.25 |
Penicillium chrysogenum |
Q12657 |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.7.1.25 |
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate |
enzyme follows a compulsory ordered mechanism in which MgATP2- binds before APS, and PAPS leaves before MgADP- |
Penicillium chrysogenum |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.1.25 |
ATP + adenosine 5-phosphosulfate |
i.e. adenylylsulfate or APS |
Penicillium chrysogenum |
ADP + 3'-phosphoadenosine 5'-phosphosulfate |
i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.25 |
dimer |
2 * 23670 |
Penicillium chrysogenum |