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Literature summary extracted from

  • Lansdon, E.B.; Segel, I.H.; Fisher, A.J.
    Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum (2002), Biochemistry, 41, 13672-13680.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.25 adenosine 5'-phosphosulfate adenosine 5'-phosphosulfate can bind to E-MgADP forming a catalytically inactive E-MgADP-APS ternary complex Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 23670
-
 2 * 23670 Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Penicillium chrysogenum Q12657
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.25 ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate enzyme follows a compulsory ordered mechanism in which MgATP2- binds before APS, and PAPS leaves before MgADP- Penicillium chrysogenum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Penicillium chrysogenum ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 23670 Penicillium chrysogenum