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Literature summary extracted from

  • Martinez-Barajas, E.; Krohn, B.M.; Stark, D.M.; Randall, D.D.
    Purification and characterization of recombinant tomato fruit (Lycopersicon esculentum Mill.) fructokinase expressed in Escherichia coli (1997), Protein Expr. Purif., 11, 41-46.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.4
-
 Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.4 ADP inhibition is dependent on fructose concentration Solanum lycopersicum
2.7.1.4 D-fructose above 5 mM Solanum lycopersicum
2.7.1.4 D-fructose 6-phosphate weak, non-competitive Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.4 0.13
-
 ATP recombinant enzyme, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.22
-
 D-fructose recombinant enzyme, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.45
-
 GTP recombinant enzyme, pH 8, 25°C Solanum lycopersicum
2.7.1.4 1.86
-
 UTP recombinant enzyme, pH 8, 25°C Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.4 35000
-
 2 * 35000, SDS-PAGE Solanum lycopersicum
2.7.1.4 72400
-
 gel filtration Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.4 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.4
-
 Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.4 fruit
-
 Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.4 22.59
-
-
 Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.4 ATP + D-fructose
-
 Solanum lycopersicum ADP + D-fructose 6-phosphate
-
 ?
2.7.1.4 GTP + D-fructose
-
 Solanum lycopersicum GDP + D-fructose 6-phosphate
-
 ?
2.7.1.4 UTP + D-fructose
-
 Solanum lycopersicum UDP + D-fructose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.4 dimer 2 * 35000, SDS-PAGE Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.4 8
-
-
 Solanum lycopersicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.4 0.12
-
 ADP at 3 mM fructose, pH 8, 25°C Solanum lycopersicum
2.7.1.4 0.41
-
 ADP at 0.5 mM fructose, pH 8, 25°C Solanum lycopersicum
2.7.1.4 3
-
 D-fructose pH 8, 25°C Solanum lycopersicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.4 Solanum lycopersicum six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE 6.17 5.66