Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Plaxton, W.C.; Storey, K.B.
    Purification and properties of aerobic and anoxic forms of pyruvate kinase from red muscle tissue of the channelled whelk, Busycotypus canaliculatum (1984), Eur. J. Biochem., 143, 257-265.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Busycotypus canaliculatum
2.7.1.40 D-fructose 2,6-diphosphate activation Busycotypus canaliculatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-oxoglutarate not Busycotypus canaliculatum
2.7.1.40 3-phosphoglycerate not Busycotypus canaliculatum
2.7.1.40 ADP at high concentrations; fructose 1,6-diphosphate partially restores Busycotypus canaliculatum
2.7.1.40 AMP MgAMP Busycotypus canaliculatum
2.7.1.40 arginine phosphate allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
2.7.1.40 ATP MgATP2- Busycotypus canaliculatum
2.7.1.40 citrate not Busycotypus canaliculatum
2.7.1.40 glucose 6-phosphate not Busycotypus canaliculatum
2.7.1.40 isocitrate not Busycotypus canaliculatum
2.7.1.40 L-alanine allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
2.7.1.40 L-lactate
-
 Busycotypus canaliculatum
2.7.1.40 L-phenylalanine alanine and fructose 1,6-diphosphate protect, kinetics; allosteric inhibitor; pH-dependent Busycotypus canaliculatum
2.7.1.40 malate not Busycotypus canaliculatum
2.7.1.40 MgATP2- allosteric inhibitor; fructose 1,6-diphosphate restores Busycotypus canaliculatum
2.7.1.40 additional information no inhibition by acetyl-CoA, NADP+, succinate, glycerol 1-phosphate, fructose 6-phosphate, D-octopine, meso-alanopine, NH4Cl, Arg, Gly, taurine, creatine phosphate Busycotypus canaliculatum
2.7.1.40 oxaloacetate not Busycotypus canaliculatum
2.7.1.40 phosphate not Busycotypus canaliculatum
2.7.1.40 proline allosteric inhibitor, fructose 1,6-diphosphate restores Busycotypus canaliculatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 additional information
-
 additional information kinetic properties Busycotypus canaliculatum
2.7.1.40 0.2 0.35 ADP pH 7.0, 20°C Busycotypus canaliculatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+
-
 Busycotypus canaliculatum
2.7.1.40 Mg2+
-
 Busycotypus canaliculatum
2.7.1.40 Mn2+ can partially replace Mg2+ Busycotypus canaliculatum
2.7.1.40 NH4+ requirement Busycotypus canaliculatum
2.7.1.40 NH4+ can replace K+ Busycotypus canaliculatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 64400
-
 4 * 64400, PK-aerobic, SDS-PAGE Busycotypus canaliculatum
2.7.1.40 232600
-
 PK-anoxic, gel filtration Busycotypus canaliculatum
2.7.1.40 237500
-
 PK-aerobic, gel filtration Busycotypus canaliculatum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Busycotypus canaliculatum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic Busycotypus canaliculatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 muscle radular retractor muscle Busycotypus canaliculatum
-
2.7.1.40 muscle red muscle Busycotypus canaliculatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Busycotypus canaliculatum
2.7.1.40 368
-
 isozyme PK-aerobic, pH 7.0, 20°C Busycotypus canaliculatum

Storage Stability

EC Number Storage Stability Organism
2.7.1.40 4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic Busycotypus canaliculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Busycotypus canaliculatum ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 64400, PK-aerobic, SDS-PAGE Busycotypus canaliculatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 additional information
-
 pH-dependence of phosphoenolpyruvate affinity Busycotypus canaliculatum
2.7.1.40 7
-
 broad Busycotypus canaliculatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 6 8 about 70% of maximal activity at pH 6 and about half-maximal activity at pH 8 Busycotypus canaliculatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.40 Busycotypus canaliculatum isozyme PK-anoxic, isoelectric focusing
-
 5.42
2.7.1.40 Busycotypus canaliculatum isozyme PK-aerobic, isoelectric focusing
-
 5.81