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Literature summary extracted from

  • Evans, C.T.; Ratledge, C.
    Partial purification and properties of pyruvate kinase and its regulatory role during lipid accumulation by the oleaginous yeast Rhodosporidium toruloides CBS 14 (1985), Can. J. Microbiol., 31, 479-484.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 D-fructose 1,6-diphosphate activation Rhodotorula toruloides
2.7.1.40 glutamic acid activation Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 AMP not Rhodotorula toruloides
2.7.1.40 ATP ADP alleviates; kinetics Rhodotorula toruloides
2.7.1.40 citrate
-
 Rhodotorula toruloides
2.7.1.40 CTP
-
 Rhodotorula toruloides
2.7.1.40 GTP
-
 Rhodotorula toruloides
2.7.1.40 isocitrate
-
 Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.12
-
 ADP
-
 Rhodotorula toruloides
2.7.1.40 0.4 0.57 phosphoenolpyruvate
-
 Rhodotorula toruloides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ requirement Rhodotorula toruloides
2.7.1.40 K+ Km-value 1.6 mM Rhodotorula toruloides
2.7.1.40 Na+ not Rhodotorula toruloides
2.7.1.40 NH4+ can replace K+ Rhodotorula toruloides
2.7.1.40 NH4+ activation Rhodotorula toruloides
2.7.1.40 NH4+ kinetics Rhodotorula toruloides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 190000
-
 gel filtration Rhodotorula toruloides

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Rhodotorula toruloides
-
-
-
2.7.1.40 Rhodotorula toruloides CBS 14
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 cell culture
-
 Rhodotorula toruloides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 additional information
-
-
 Rhodotorula toruloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Rhodotorula toruloides ATP + pyruvate
-
 ?
2.7.1.40 ADP + phosphoenolpyruvate
-
 Rhodotorula toruloides CBS 14 ATP + pyruvate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 8
-
-
 Rhodotorula toruloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.40 7.6 8.2
-
 Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 AMP not Rhodotorula toruloides