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Literature summary extracted from

  • Neuberger, M.S.; Hartley, B.S.; Walker, J.E.
    Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes (1981), Biochem. J., 193, 513-524.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 expressed in Escherichia coli K12 strain NC260 harbouring multi-copy lambdaprbt-101 dal-201 prophage Klebsiella aerogenes
2.7.1.47 expression in Escherichia coli K12 strain NC629 harbouring multi-copy lambdaprbt-101 prophage which carries the rbt-operon Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.8
-
 D-xylulose
-
 Klebsiella aerogenes
2.7.1.47 0.4
-
 D-ribulose pH 7.8, 28°C Klebsiella aerogenes
2.7.1.47 140
-
 D-arabitol pH 7.8, 28°C Klebsiella aerogenes
2.7.1.47 220
-
 ribitol pH 7.8, 28°C Klebsiella aerogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.17 cytosol
-
 Klebsiella aerogenes 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 54000
-
 2 * 54000, SDS-PAGE Klebsiella aerogenes
2.7.1.17 110000
-
 gel filtration Klebsiella aerogenes
2.7.1.47 60000
-
 2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE Klebsiella aerogenes
2.7.1.47 112000
-
 recombinant enzyme expressed in E. coli strain NC629, gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Klebsiella aerogenes
-
 expressed as constitutive enzyme in Escherichia coli K12 construct strain NC260
-
2.7.1.47 Klebsiella aerogenes
-
 expressed as constitutive enzyme in Escherichia coli K12 construct strain NC629
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.17
-
 Klebsiella aerogenes
2.7.1.47
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.47 84
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.47 -20°C, in 50% v/v glycerol, in the dark, 1 month Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 ATP + D-xylulose
-
 Klebsiella aerogenes ADP + D-xylulose 5-phosphate
-
 r
2.7.1.47 ATP + D-arabitol poor substrate, not L-isomer Klebsiella aerogenes ?
-
 ?
2.7.1.47 ATP + D-ribulose
-
 Klebsiella aerogenes ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.47 ATP + ribitol poor substrate Klebsiella aerogenes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.17 dimer 2 * 54000, SDS-PAGE Klebsiella aerogenes
2.7.1.47 dimer 2 * 60000, recombinant enzyme expressed in Escherichia coli strain NC629, SDS-PAGE Klebsiella aerogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.17 4
-
 inactivation, only partially reversible by incubation at 37°C Klebsiella aerogenes