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Literature summary extracted from

  • Kruger, N.J.; Dennis, D.T.
    Molecular properties of pyrophosphate:D-fructose-6-phosphate phosphotransferase from potato tuber (1987), Arch. Biochem. Biophys., 256, 273-279.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ requirement Triticum aestivum
2.7.1.90 Mg2+ requirement Spinacia oleracea
2.7.1.90 Mg2+ requirement Pisum sativum
2.7.1.90 Mg2+ requirement Zea mays
2.7.1.90 Mg2+ requirement Solanum tuberosum
2.7.1.90 Mg2+ requirement Ricinus communis
2.7.1.90 Mg2+ requirement Vigna radiata
2.7.1.90 Mg2+ requirement Daucus carota
2.7.1.90 Mg2+ requirement Brassica oleracea
2.7.1.90 Mg2+ requirement Allium cepa
2.7.1.90 Mg2+ requirement Persea americana
2.7.1.90 Mg2+ requirement Acer saccharum
2.7.1.90 Mg2+ requirement Kalanchoe blossfeldiana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 60000
-
 2 * 65000 + 2 * 60000, SDS-PAGE Solanum tuberosum
2.7.1.90 65000
-
 2 * 65000 + 2 * 60000, SDS-PAGE Solanum tuberosum
2.7.1.90 265000
-
 gel filtration, in the presence of diphosphate the enzyme dissociates into a 130000 dimer, D-fructose 2,6-bisphosphate prevents or reassociates Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Triticum aestivum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Spinacia oleracea
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Pisum sativum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Zea mays
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Solanum tuberosum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Ricinus communis
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Vigna radiata
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Daucus carota
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Brassica oleracea
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Allium cepa
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Persea americana
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Acer saccharum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Kalanchoe blossfeldiana
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Acer saccharum
-
-
-
2.7.1.90 Allium cepa
-
-
-
2.7.1.90 Brassica oleracea
-
 var. botrytis
-
2.7.1.90 Daucus carota
-
-
-
2.7.1.90 Kalanchoe blossfeldiana
-
-
-
2.7.1.90 Persea americana
-
-
-
2.7.1.90 Pisum sativum
-
-
-
2.7.1.90 Ricinus communis
-
 var. Hale
-
2.7.1.90 Solanum tuberosum
-
-
-
2.7.1.90 Spinacia oleracea
-
-
-
2.7.1.90 Triticum aestivum
-
-
-
2.7.1.90 Vigna radiata
-
 mung bean
-
2.7.1.90 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial Solanum tuberosum
2.7.1.90 to homogeneity Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 bulb
-
 Allium cepa
-
2.7.1.90 cotyledon
-
 Pisum sativum
-
2.7.1.90 cotyledon
-
 Acer saccharum
-
2.7.1.90 endosperm
-
 Triticum aestivum
-
2.7.1.90 endosperm
-
 Ricinus communis
-
2.7.1.90 floral primordium differentiated Brassica oleracea
-
2.7.1.90 hypocotyl
-
 Vigna radiata
-
2.7.1.90 leaf
-
 Triticum aestivum
-
2.7.1.90 leaf
-
 Spinacia oleracea
-
2.7.1.90 leaf
-
 Zea mays
-
2.7.1.90 leaf
-
 Ricinus communis
-
2.7.1.90 leaf
-
 Kalanchoe blossfeldiana
-
2.7.1.90 mesocarp
-
 Persea americana
-
2.7.1.90 radicle
-
 Pisum sativum
-
2.7.1.90 root tap root Daucus carota
-
2.7.1.90 tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 10
-
 purified enzyme Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Triticum aestivum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Spinacia oleracea phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Pisum sativum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Pisum sativum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Zea mays phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Zea mays phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Solanum tuberosum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Ricinus communis phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Vigna radiata phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Daucus carota phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Brassica oleracea phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Brassica oleracea phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Allium cepa phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Allium cepa phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Persea americana phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Persea americana phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Acer saccharum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Acer saccharum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Kalanchoe blossfeldiana phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Kalanchoe blossfeldiana phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Triticum aestivum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Spinacia oleracea phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Solanum tuberosum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Ricinus communis phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Vigna radiata phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Daucus carota phosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 tetramer
-
 Daucus carota
2.7.1.90 tetramer 2 * 65000 + 2 * 60000, SDS-PAGE Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Spinacia oleracea
2.7.1.90 25
-
 assay at Solanum tuberosum
2.7.1.90 25
-
 assay at Ricinus communis
2.7.1.90 25
-
 assay at Daucus carota