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Literature summary extracted from

  • Krishna, R.V.; Leisinger, T.
    Biosynthesis of proline in Pseudomonas aeruginosa. Partial purification and characterization of gamma-glutamyl kinase (1979), Biochem. J., 181, 215-222.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.11 5-oxo-L-Pro 12 mM, 10% inhibition Pseudomonas aeruginosa
2.7.2.11 ADP
-
 Pseudomonas aeruginosa
2.7.2.11 Cd2+ 0.1 mM, complete inhibition Pseudomonas aeruginosa
2.7.2.11 DL-3,4-Didehydroproline 9 mM, 50% inhibition Pseudomonas aeruginosa
2.7.2.11 Hg2+ 0.1 mM, complete inhibition Pseudomonas aeruginosa
2.7.2.11 iodoacetamide 0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
2.7.2.11 L-azetidine-2-carboxylic acid 3 mM, 50% inhibition Pseudomonas aeruginosa
2.7.2.11 L-methionine-DL-sulphoximine competitive with L-glutamate Pseudomonas aeruginosa
2.7.2.11 L-Orn 12 mM, 10% inhibition Pseudomonas aeruginosa
2.7.2.11 L-Pro enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase Pseudomonas aeruginosa
2.7.2.11 L-Thioproline 12 mM, 10% inhibition Pseudomonas aeruginosa
2.7.2.11 Mg2+ above 20 mM Pseudomonas aeruginosa
2.7.2.11 Mn2+ above 20 mM Pseudomonas aeruginosa
2.7.2.11 NEM 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
2.7.2.11 PCMB 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects Pseudomonas aeruginosa
2.7.2.11 phosphate
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.11 12
-
 L-glutamate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.11 Mg2+ required Pseudomonas aeruginosa
2.7.2.11 Mn2+ can partially replace Mg2+ Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.11 84000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.11 Pseudomonas aeruginosa
-
-
-
2.7.2.11 Pseudomonas aeruginosa PAO 1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.11 partial Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.11 12.68
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
2.7.2.11 -40°C, 40% loss of activity within 2 months, then remains stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.11 ATP + 5-ethyl-L-glutamate 5% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
 ?
2.7.2.11 ATP + 5-ethyl-L-glutamate 5% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
 ?
2.7.2.11 ATP + 5-methyl-L-glutamate 6% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
 ?
2.7.2.11 ATP + 5-methyl-L-glutamate 6% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
 ?
2.7.2.11 ATP + L-glutamate
-
 Pseudomonas aeruginosa ADP + L-glutamate 5-phosphate
-
 ?
2.7.2.11 ATP + L-glutamate
-
 Pseudomonas aeruginosa PAO 1 ADP + L-glutamate 5-phosphate
-
 ?
2.7.2.11 ATP + L-glutamine 10% of the activity with L-glutamate Pseudomonas aeruginosa ?
-
 ?
2.7.2.11 ATP + L-glutamine 10% of the activity with L-glutamate Pseudomonas aeruginosa PAO 1 ?
-
 ?
2.7.2.11 GTP + L-glutamate 10% of the activity with ATP Pseudomonas aeruginosa GDP + L-glutamate 5-phosphate
-
 ?
2.7.2.11 GTP + L-glutamate 10% of the activity with ATP Pseudomonas aeruginosa PAO 1 GDP + L-glutamate 5-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.11 6 6.3
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.11 5.5 8.5 pH 5.5: about 80% of maximal activity, pH 8.5: about 40% of maximal activity Pseudomonas aeruginosa