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Literature summary extracted from

  • Kuntz, G.W.K.; Krietsch, W.K.G.
    Phosphoglycerate kinase from animal tissue (1982), Methods Enzymol., 90, 103-110.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.2.3 bovine serum albumin and dithioerythritol stabilize in the enzyme assay Mus musculus
2.7.2.3 bovine serum albumin and dithioerythritol stabilize in the enzyme assay Homo sapiens
2.7.2.3 bovine serum albumin and dithioerythritol stabilize in the enzyme assay Oryctolagus cuniculus
2.7.2.3 bovine serum albumin and dithioerythritol stabilize in the enzyme assay Equus caballus
2.7.2.3 bovine serum albumin and dithioerythritol stabilize in the enzyme assay trout

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 2,3-diphosphoglycerate competitive to 3-phospho-D-glycerate and MgATP2- Equus caballus
2.7.2.3 2,3-diphosphoglycerate competitive to 3-phospho-D-glycerate and MgATP2- Homo sapiens
2.7.2.3 2,3-diphosphoglycerate competitive to 3-phospho-D-glycerate and MgATP2- Mus musculus
2.7.2.3 2,3-diphosphoglycerate competitive to 3-phospho-D-glycerate and MgATP2- Oryctolagus cuniculus
2.7.2.3 2,3-diphosphoglycerate competitive to 3-phospho-D-glycerate and MgATP2- trout
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Equus caballus
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Homo sapiens
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Mus musculus
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Oryctolagus cuniculus
2.7.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 trout
2.7.2.3 AMP
-
 Equus caballus
2.7.2.3 AMP
-
 Homo sapiens
2.7.2.3 AMP
-
 Mus musculus
2.7.2.3 AMP
-
 Oryctolagus cuniculus
2.7.2.3 AMP
-
 trout
2.7.2.3 GMP
-
 Equus caballus
2.7.2.3 GMP
-
 Homo sapiens
2.7.2.3 GMP
-
 Mus musculus
2.7.2.3 GMP
-
 Oryctolagus cuniculus
2.7.2.3 GMP
-
 trout
2.7.2.3 IMP
-
 Equus caballus
2.7.2.3 IMP
-
 Homo sapiens
2.7.2.3 IMP
-
 Mus musculus
2.7.2.3 IMP
-
 Oryctolagus cuniculus
2.7.2.3 IMP
-
 trout

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information kinetics Mus musculus
2.7.2.3 additional information
-
 additional information kinetics Homo sapiens
2.7.2.3 additional information
-
 additional information kinetics Oryctolagus cuniculus
2.7.2.3 additional information
-
 additional information kinetics Equus caballus
2.7.2.3 additional information
-
 additional information kinetics trout

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.3 cytosol
-
 Mus musculus 5829
-
2.7.2.3 cytosol
-
 Homo sapiens 5829
-
2.7.2.3 cytosol
-
 Oryctolagus cuniculus 5829
-
2.7.2.3 cytosol
-
 trout 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 44520
-
 amino acid sequence Equus caballus
2.7.2.3 44660
-
 erythrocytes, amino acid sequence Homo sapiens
2.7.2.3 46000
-
 1 * 46000, SDS-PAGE Homo sapiens
2.7.2.3 46000
-
 1 * 46000, SDS-PAGE Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Equus caballus
-
-
-
2.7.2.3 Homo sapiens
-
-
-
2.7.2.3 Mus musculus
-
-
-
2.7.2.3 Oryctolagus cuniculus
-
-
-
2.7.2.3 trout
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 101fold from skeletal muscle, 3780fold from erythrocytes Homo sapiens
2.7.2.3 135fold trout
2.7.2.3 70fold Oryctolagus cuniculus
2.7.2.3 99fold from muscle, 333fold from testis Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 erythrocyte
-
 Homo sapiens
-
2.7.2.3 muscle
-
 Mus musculus
-
2.7.2.3 muscle
-
 Homo sapiens
-
2.7.2.3 muscle
-
 Oryctolagus cuniculus
-
2.7.2.3 muscle
-
 Equus caballus
-
2.7.2.3 muscle
-
 trout
-
2.7.2.3 skeletal muscle
-
 Homo sapiens
-
2.7.2.3 skeletal muscle
-
 Oryctolagus cuniculus
-
2.7.2.3 skeletal muscle
-
 Equus caballus
-
2.7.2.3 testis
-
 Mus musculus
-
2.7.2.3 testis
-
 Homo sapiens
-
2.7.2.3 testis
-
 Oryctolagus cuniculus
-
2.7.2.3 testis
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 600
-
 purified testis enzyme Mus musculus
2.7.2.3 680
-
 purified erythrocyte enzyme Homo sapiens
2.7.2.3 695
-
 purified enzyme Oryctolagus cuniculus
2.7.2.3 700
-
-
 Oryctolagus cuniculus
2.7.2.3 700
-
-
 Equus caballus
2.7.2.3 700
-
 purified enzyme trout
2.7.2.3 710
-
 purified enzyme Mus musculus
2.7.2.3 710
-
 purified muscle enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years Mus musculus
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years Oryctolagus cuniculus
2.7.2.3 as a precipitate in 3.3 M ammonium sulfate, stable for years trout

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Homo sapiens ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Oryctolagus cuniculus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Equus caballus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 trout ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Mus musculus CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Homo sapiens CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate no activity Oryctolagus cuniculus CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate no activity trout CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate
-
 Mus musculus dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate
-
 Equus caballus dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dGTP + 3-phospho-D-glycerate
-
 Mus musculus dGDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dGTP + 3-phospho-D-glycerate
-
 Equus caballus dGDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Mus musculus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Homo sapiens GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Oryctolagus cuniculus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 Equus caballus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate
-
 trout GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Mus musculus IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Homo sapiens IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Oryctolagus cuniculus IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 Equus caballus IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 ITP + 3-phospho-D-glycerate
-
 trout IDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 additional information the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid Mus musculus ?
-
 ?
2.7.2.3 additional information the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid Homo sapiens ?
-
 ?
2.7.2.3 additional information the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid Oryctolagus cuniculus ?
-
 ?
2.7.2.3 additional information the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid Equus caballus ?
-
 ?
2.7.2.3 additional information the only analog of 3-phospho-D-glycerate that can replace the substrate is an artificial 3-phospho-D-glycerate in which the phosphate group-O-PO3H2 is replaced by the phosphomethyl group-CH2-PO3H2, i.e. 2-hydroxy-4-phospho-DL-butyric acid trout ?
-
 ?
2.7.2.3 additional information nucleotide substrate specificity Mus musculus ?
-
 ?
2.7.2.3 additional information nucleotide substrate specificity Homo sapiens ?
-
 ?
2.7.2.3 additional information nucleotide substrate specificity Oryctolagus cuniculus ?
-
 ?
2.7.2.3 additional information nucleotide substrate specificity trout ?
-
 ?
2.7.2.3 additional information no activity with dTTP Mus musculus ?
-
 ?
2.7.2.3 additional information no activity with dTTP Homo sapiens ?
-
 ?
2.7.2.3 additional information no activity with dTTP Oryctolagus cuniculus ?
-
 ?
2.7.2.3 additional information no activity with dTTP trout ?
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 Mus musculus UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 Homo sapiens UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 Oryctolagus cuniculus UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 Equus caballus UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate
-
 trout UDP + 1,3-diphosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer
-
 Mus musculus
2.7.2.3 monomer
-
 Oryctolagus cuniculus
2.7.2.3 monomer
-
 trout
2.7.2.3 monomer 1 * 46000, SDS-PAGE Homo sapiens
2.7.2.3 monomer 1 * 46000, SDS-PAGE Equus caballus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Mus musculus
2.7.2.3 25
-
 assay at Homo sapiens
2.7.2.3 25
-
 assay at Oryctolagus cuniculus
2.7.2.3 25
-
 assay at Equus caballus
2.7.2.3 25
-
 assay at trout

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 6 9
-
 trout
2.7.2.3 6 8.5
-
 Homo sapiens
2.7.2.3 6 9.3
-
 Oryctolagus cuniculus
2.7.2.3 6.5 8.5
-
 Mus musculus
2.7.2.3 7.6
-
 assay at Mus musculus
2.7.2.3 7.6
-
 assay at Homo sapiens
2.7.2.3 7.6
-
 assay at Oryctolagus cuniculus
2.7.2.3 7.6
-
 assay at Equus caballus
2.7.2.3 7.6
-
 assay at trout

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Mus musculus
2.7.2.3 ADP
-
 Homo sapiens
2.7.2.3 ADP
-
 Oryctolagus cuniculus
2.7.2.3 ADP
-
 Equus caballus
2.7.2.3 ADP
-
 trout
2.7.2.3 ATP required as phosphate donor Mus musculus
2.7.2.3 ATP required as phosphate donor Homo sapiens
2.7.2.3 ATP required as phosphate donor Oryctolagus cuniculus
2.7.2.3 ATP required as phosphate donor Equus caballus
2.7.2.3 ATP required as phosphate donor trout
2.7.2.3 dATP
-
 Mus musculus
2.7.2.3 dATP
-
 Equus caballus
2.7.2.3 dGTP
-
 Mus musculus
2.7.2.3 dGTP
-
 Equus caballus
2.7.2.3 GTP
-
 Mus musculus
2.7.2.3 GTP
-
 Homo sapiens
2.7.2.3 GTP
-
 Oryctolagus cuniculus
2.7.2.3 GTP
-
 Equus caballus
2.7.2.3 GTP
-
 trout
2.7.2.3 ITP
-
 Mus musculus
2.7.2.3 ITP
-
 Homo sapiens
2.7.2.3 ITP
-
 Oryctolagus cuniculus
2.7.2.3 ITP
-
 Equus caballus
2.7.2.3 ITP
-
 trout
2.7.2.3 UTP
-
 Mus musculus
2.7.2.3 UTP
-
 Homo sapiens
2.7.2.3 UTP
-
 Oryctolagus cuniculus
2.7.2.3 UTP
-
 Equus caballus
2.7.2.3 UTP
-
 trout

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.3 2.3
-
 2,3-diphosphoglycerate versus MgATP2- Mus musculus
2.7.2.3 2.3
-
 2,3-diphosphoglycerate versus MgATP2- Homo sapiens
2.7.2.3 2.3
-
 2,3-diphosphoglycerate versus MgATP2- Oryctolagus cuniculus
2.7.2.3 2.3
-
 2,3-diphosphoglycerate versus MgATP2- Equus caballus
2.7.2.3 2.3
-
 2,3-diphosphoglycerate versus MgATP2- trout
2.7.2.3 3.8
-
 2,3-diphosphoglycerate versus 3-phospho-D-glycerate Mus musculus
2.7.2.3 3.8
-
 2,3-diphosphoglycerate versus 3-phospho-D-glycerate Homo sapiens
2.7.2.3 3.8
-
 2,3-diphosphoglycerate versus 3-phospho-D-glycerate Oryctolagus cuniculus
2.7.2.3 3.8
-
 2,3-diphosphoglycerate versus 3-phospho-D-glycerate Equus caballus
2.7.2.3 3.8
-
 2,3-diphosphoglycerate versus 3-phospho-D-glycerate trout