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Literature summary extracted from

  • Chiga, M.; Rogers, A.E.; Plaut, G.W.E.
    Nucleotide transphosphorylases from liver (1961), J. Biol. Chem., 236, 1800-1805.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.10 AgNO3 weak Sus scrofa
2.7.4.10 HgCl2 weak Sus scrofa
2.7.4.10 N-ethylmaleimide weak Sus scrofa
2.7.4.10 p-hydroxymercuribenzoate weak Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.10 Mg2+ actual substrate: MgNTP Sus scrofa
2.7.4.10 Mn2+ 75% as effective as Mg2+ Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.10 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.10
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.10 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.10 17.5
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.4.10 -20°C to 4°C, a few weeks Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.10 dGTP + dAMP
-
 Sus scrofa dGDP + dADP
-
 r
2.7.4.10 GTP + AMP no acceptor substrates are 2'-AMP, 3'-AMP Sus scrofa GDP + ADP
-
 r
2.7.4.10 GTP + AMP not: CMP, GMP, IMP, UMP Sus scrofa GDP + ADP
-
 r
2.7.4.10 GTP + AMP dAMP can replace AMP Sus scrofa GDP + ADP
-
 r
2.7.4.10 ITP + AMP dAMP can replace AMP Sus scrofa IDP + ADP
-
 r
2.7.4.10 ITP + AMP not: dCMP, dGMP, TDP, deoxyadenosine Sus scrofa IDP + ADP
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.10 25
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.10
-
-
 5 min, 2 mg protein/ml, stable in 0.1 N HCl Sus scrofa
2.7.4.10 40
-
 5 min, 2 mg protein/ml, in 0.1 N HCl, 70% loss of activity Sus scrofa
2.7.4.10 95
-
 5 min, 95% inactivation at pH 7.4, 0.15 M imidazole buffer Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.10 7.4
-
 IDP, ADP Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.10 6 9 about half-maximal activity at pH 6 and about 80% of maximal activity at pH 9 Sus scrofa
2.7.4.10 6 9 at pH 4.5 the activity is about 2% of that at pH 7.4 Sus scrofa