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Literature summary extracted from

  • Itakura, T.; Watanabe, K.; Shiokawa, H.; Kubo, S.
    Purification and characterization of acidic adenylate kinase in porcine heart (1978), Eur. J. Biochem., 82, 431-437.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 5,5'-dithiobis(2-nitrobenzoic acid) not Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 Mg2+ requirement Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 26900
-
 acidic isozyme, PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Sus scrofa
-
 acidic adenylate kinase from pig heart resembles liver mitochondrial enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3 acidic isozyme Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.3 ATP + AMP = 2 ADP mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.3 heart
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
2.7.4.3 -80°C, 22 mM Tris-HCl, 0.5 M NaCl, pH 7.8, a few months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP + ADP
-
 Sus scrofa ATP + AMP
-
 r
2.7.4.3 ATP + AMP
-
 Sus scrofa ADP + ADP
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.3 5.8
-
 AMP + ATP, acidic heart enzyme Sus scrofa
2.7.4.3 7 8 muscle enzyme Sus scrofa
2.7.4.3 7
-
 ADP + ADP, acidic heart enzyme Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.3 5 7.5 about half-maximal activity at pH 5 and 7.5, AMP + ATP, acidic heart enzyme Sus scrofa
2.7.4.3 6 8.5 about half-maximal activity at pH 6 and 8.5, ADP + ADP, acidic heart enzyme Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.3 3
-
 2 h, at 25°C, 75% loss of activity, stable at neutral pH-values, acidic heart enzyme Sus scrofa