Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chowdhury, R.R.
    Purification & some properties of galactose-1-phosphate uridylyl transferase from E. coli (1979), Indian J. Biochem. Biophys., 16, 273-277.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.12 2-mercaptoethanol 2fold at 10 mM Escherichia coli
2.7.7.12 cysteine activation Escherichia coli
2.7.7.12 cysteine requirement Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.12 DTNB binds to SH-group in the active center Escherichia coli
2.7.7.12 NEM complete inhibition at 0.05 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.12 41000
-
 2 * 41000 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.12 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.12 over 200fold Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.12 UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose SH-group in the active site Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.12 UDP-glucose + alpha-D-galactose 1-phosphate
-
 Escherichia coli alpha-D-glucose 1-phosphate + UDP-galactose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.12 dimer 2 * 41000 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.12 8.5
-
-
 Escherichia coli