EC Number | General Stability | Organism |
---|---|---|
2.7.7.4 | 5°C, enzyme solution with 5-10 mg/ml, stable for several months | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.4 | ADP | linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP | Rattus norvegicus | |
2.7.7.4 | AMP | linear competitive inhibitor with respect to SO42-, uncompetitive with respect to ATP | Rattus norvegicus | |
2.7.7.4 | ATP | product inhibitor in formation of ATP from diphosphate and adenylylsulfate | Rattus norvegicus | |
2.7.7.4 | ClO3- | - |
Rattus norvegicus | |
2.7.7.4 | ClO4- | linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP | Rattus norvegicus | |
2.7.7.4 | EDTA | inhibits due to chelation of Mg2+ | Rattus norvegicus | |
2.7.7.4 | NEM | 10 mM, inhibits reaction with diphosphate and adenylylsulfate | Rattus norvegicus | |
2.7.7.4 | NO3- | linear competitive inhibitor with respect to SO42- and uncompetitive with respect to ATP | Rattus norvegicus | |
2.7.7.4 | PCMB | 5 mM, inhibits reaction with diphosphate and adenylylsulfate | Rattus norvegicus | |
2.7.7.4 | SeO42- | - |
Rattus norvegicus | |
2.7.7.4 | SO42- | product inhibitor in formation of ATP from diphosphate and adenylylsulfate | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 0.018 | - |
diphosphate | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.025 | - |
adenylylsulfate | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.38 | - |
ATP | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.61 | - |
SeO42- | pH 7.8, 37°C, SeO42dependent ATP-diphosphate exchange reaction | Rattus norvegicus | |
2.7.7.4 | 2.5 | - |
SO42- | pH 7.8, 37°C, exchange reaction | Rattus norvegicus | |
2.7.7.4 | 3.2 | - |
SO42- | pH 7.8, 37°C, formation of adenylylsulfate | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.4 | Mg2+ | formation of ATP from diphosphate and adenylylsulfate is absolutely dependent upon the presence of Mg2+ | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 410000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.4 | Rattus norvegicus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.7.7.4 | not stable to freezing and thawing | Rattus norvegicus |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.7.4 | lipoprotein | - |
Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.4 | - |
Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.7.4 | ATP + sulfate = diphosphate + adenylyl sulfate | sequential bi-bi reaction with ATP being the first substrate bound and adenylysulfate the last product released | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.4 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 2.1 | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.4 | ATP + SeO42- | - |
Rattus norvegicus | AMP + adenylylselenate | - |
? | |
2.7.7.4 | ATP + sulfate | - |
Rattus norvegicus | diphosphate + adenylylsulfate | - |
r | |
2.7.7.4 | additional information | enzyme catalyzes ATP-diphosphate exchange reaction. The enzyme does notto catalyze the incorporation of diphosphate into ATP in the absence of SO42-. The enzyme catalyzes SeO42dependent ATP-diphosphate exchange | Rattus norvegicus | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 7.8 | 8 | 0.1 M phosphate buffer | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.4 | 7.8 | - |
unstable in Tris, diethylbarbiturate, glycine, triethanolamine and bicarbonate buffer, but stable in phosphate buffers of the same pH | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.4 | 0.06 | - |
ADP | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.25 | - |
ClO3- | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.5 | - |
SeO42- | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 0.6 | - |
AMP | pH 7.8, 37°C | Rattus norvegicus | |
2.7.7.4 | 2.2 | - |
NO3- | pH 7.8, 37°C | Rattus norvegicus |