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Literature summary extracted from

  • Shoyab, M.; Su, L.Y.; Marx, W.
    Purification and properties of ATP-sulfurylase from Furth mouse mastocytoma (1972), Biochim. Biophys. Acta, 258, 113-124.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.4 freezing and thawing inactivates the purified enzyme Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.4 Ba2+ 2 mM Mus musculus
2.7.7.4 Ca2+ 2 mM Mus musculus
2.7.7.4 deoxyadenylylsulfate 1 mM, in presence of about 20% inhibition Mus musculus
2.7.7.4 EDTA inhibition is reversed by mn2+, Mg2+, Cu2+, Co2+ Mus musculus
2.7.7.4 guanylylsulfate 1 mM, in presence of adenylylsulfate about 20% inhibition Mus musculus
2.7.7.4 inosylylsulfate 1 mM, in presence of adenylylsulfate about 20% inhibition Mus musculus
2.7.7.4 Ni2+ 2 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 additional information
-
 additional information the reaction does not strictly follow Michaelis-Menten kinetics Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Mg2+ no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ Mus musculus
2.7.7.4 Mn2+ no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ Mus musculus
2.7.7.4 Zn2+ no absolute requirement for metal ions, but activity is increased by Mn2+, Mg2+ and Zn2+ Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.4 mastocytoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 9.3
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.7.7.4 0-4°C, purified enzyme solution, 1 mg protein per ml, 15% loss of activity after 5 weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Mus musculus diphosphate + adenylylsulfate
-
 r
2.7.7.4 dATP + SO42-
-
 Mus musculus diphosphate + deoxyadenylylsulfate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8.5
-
-
 Mus musculus