Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Karamohamed, S.; Nilsson, J.; Nourizad, K.; Ronaghi, M.; Pettersson, B.; Nyren, P.
    Production, Purification, and Luminometric Analysis of Recombinant Saccharomyces cerevisiae MET3 Adenosine Triphosphate Sulfurylase Expressed in Escherichia coli (1999), Protein Expr. Purif., 15, 381-388.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.4 ATP sulfurylase cDNA from MET3 on chromosome X is amplified and expressed in Escherichia coli XL1-Blue. The synthesis of the enzyme is directed by an expression system that employs the regulatory genes of Vibrio fischeri Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 59300
-
 8 * 59300, SDS-PAGE Saccharomyces cerevisiae
2.7.7.4 470000
-
 recombinant enzyme, gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4 recombinant enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 140
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Saccharomyces cerevisiae diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 octamer 8 * 59300, SDS-PAGE Saccharomyces cerevisiae