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Literature summary extracted from

  • Onda, M.; Morimoto, A.; Simoide, A.; Iwata, K.; Nakajima, H.
    Purification and properties of adenosine 5'-triphosphate sulfurylase from the thermophilic bacterium Bacillus stearothermophilus (1996), Biosci. Biotechnol. Biochem., 60, 1740-1742.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.4 0.045
-
 ATP pH 6.0, 40°C Geobacillus stearothermophilus
2.7.7.4 0.16
-
 dATP pH 6.0, 40°C Geobacillus stearothermophilus
2.7.7.4 0.2
-
 SO42- pH 6.0, 40°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Co2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus
2.7.7.4 Mg2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus
2.7.7.4 Mn2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 44000
-
 2 * 44000, SDS-PAGE Geobacillus stearothermophilus
2.7.7.4 100000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 ATP + sulfate Geobacillus stearothermophilus the enzyme catalyzes the first step of sulfate activation diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate Geobacillus stearothermophilus NCA 1503 the enzyme catalyzes the first step of sulfate activation diphosphate + adenylylsulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Geobacillus stearothermophilus
-
-
-
2.7.7.4 Geobacillus stearothermophilus NCA 1503
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 7.04
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Geobacillus stearothermophilus diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate the enzyme catalyzes the first step of sulfate activation Geobacillus stearothermophilus diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Geobacillus stearothermophilus NCA 1503 diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate the enzyme catalyzes the first step of sulfate activation Geobacillus stearothermophilus NCA 1503 diphosphate + adenylylsulfate
-
 r
2.7.7.4 dATP + SO42-
-
 Geobacillus stearothermophilus diphosphate + deoxyadenylylsulfate
-
 ?
2.7.7.4 dATP + SO42-
-
 Geobacillus stearothermophilus NCA 1503 diphosphate + deoxyadenylylsulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.4 dimer 2 * 44000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.4 70
-
 15 min, stable below. 20% loss of activity after 1 h Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.4 8.5 8.7
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.4 7.2 9.1 pH 7.2: about 45% of maximal activity, pH 9.1: about 50% of maximal activity Geobacillus stearothermophilus