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Literature summary extracted from

  • Gavel, O.Y.; Bursakov, S.A.; Calvete, J.J.; George, G.N.; Moura, J.J.G.; Moura, I.
    ATP Sulfurylases from sulfate-reducing bacteria of the genus Desulfovibrio. A novel metalloprotein containing cobalt and zZinc (1998), Biochemistry, 37, 16225-16232.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.4 Cobalt metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms Desulfovibrio desulfuricans
2.7.7.4 Cobalt metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms Megalodesulfovibrio gigas
2.7.7.4 Cobalt contains 1.53 mol of zinc per mol of protein Megalodesulfovibrio gigas
2.7.7.4 Cobalt contains 1.89 mol of zinc per mol of protein Desulfovibrio desulfuricans
2.7.7.4 Zinc contains 0.72 mol of zinc per mol of protein Desulfovibrio desulfuricans
2.7.7.4 Zinc contains 1.38 mol of zinc per mol of protein Megalodesulfovibrio gigas
2.7.7.4 Zinc metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms Desulfovibrio desulfuricans
2.7.7.4 Zinc metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms Megalodesulfovibrio gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.4 47100
-
 3 * 47100, electrospray mass spectrometry Desulfovibrio desulfuricans
2.7.7.4 138000
-
 gel filtration Desulfovibrio desulfuricans
2.7.7.4 151000
-
 gel filtration Megalodesulfovibrio gigas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.4 ATP + sulfate Desulfovibrio desulfuricans
-
 diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate Megalodesulfovibrio gigas enzyme plays a crucial role in sulfate activation diphosphate + adenylylsulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.4 Desulfovibrio desulfuricans
-
 ATCC 27774
-
2.7.7.4 Megalodesulfovibrio gigas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.4
-
 Desulfovibrio desulfuricans
2.7.7.4
-
 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.4 22.5
-
-
 Desulfovibrio desulfuricans
2.7.7.4 28.9
-
-
 Megalodesulfovibrio gigas

Storage Stability

EC Number Storage Stability Organism
2.7.7.4 4°C, 50 mM MOPS buffer, pH 7.4, 0.2 mM CoCl2, 0.2 mM ZnCl2, stable for 48 h Desulfovibrio desulfuricans
2.7.7.4 4°C, 50 mM MOPS buffer, pH 7.4, 0.2 mM CoCl2, 0.2 mM ZnCl2, stable for 48 h Megalodesulfovibrio gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.4 ATP + sulfate
-
 Desulfovibrio desulfuricans diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate
-
 Megalodesulfovibrio gigas diphosphate + adenylylsulfate
-
 r
2.7.7.4 ATP + sulfate enzyme plays a crucial role in sulfate activation Megalodesulfovibrio gigas diphosphate + adenylylsulfate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.4 trimer 3 * 47100, electrospray mass spectrometry Desulfovibrio desulfuricans
2.7.7.4 trimer 3 * 48000-50000, electrospray mass spectrometry Megalodesulfovibrio gigas