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Literature summary extracted from

  • Bias, U.; Trüper, H.G.
    Species specific release of sulfate from adenylyl sulfate by ATP sulfurylase or ADP sulfurylase in the green sulfur bacteria Chlorobium limicola and Chlorobium vibrioforme (1987), Arch. Microbiol., 147, 406-410.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.5 very labile , 50% loss of activity by four times freeze and thaw Prosthecochloris vibrioformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.5 phosphate above 50 mM Prosthecochloris vibrioformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.5 0.28
-
 adenosine 5'-phosphosulfate pH 8.0, 50 mM phosphate Prosthecochloris vibrioformis
2.7.7.5 8
-
 phosphate pH 8, 1 mM adenosine 5'-phosphosulfate Prosthecochloris vibrioformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.5 120000
-
 gel filtration Prosthecochloris vibrioformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.5 phosphate + adenosine 5'-phosphosulfate Prosthecochloris vibrioformis
-
 ADP + SO42-
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate Prosthecochloris vibrioformis 8327
-
 ADP + SO42-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.5 Prosthecochloris vibrioformis
-
-
-
2.7.7.5 Prosthecochloris vibrioformis 8327
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.5 -18°C stable for several weeks Prosthecochloris vibrioformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.5 phosphate + adenosine 5'-phosphosulfate
-
 Prosthecochloris vibrioformis ADP + SO42-
-
 ?
2.7.7.5 phosphate + adenosine 5'-phosphosulfate
-
 Prosthecochloris vibrioformis 8327 ADP + SO42-
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.5 60
-
 69% loss of activity within 5 min Prosthecochloris vibrioformis
2.7.7.5 70
-
 no activity after 5 min Prosthecochloris vibrioformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.5 8.5 9 slight differences in different buffer systems Prosthecochloris vibrioformis