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Literature summary extracted from

  • Kelly, K.O.; Deutscher, M.P.
    Characterization of Escherichia coli RNase PH (1992), J. Biol. Chem., 267, 17153-17158.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.56 EDTA
-
Escherichia coli
2.7.7.56 N-ethylmaleimide
-
Escherichia coli
2.7.7.56 p-hydroxymercuribenzoate
-
Escherichia coli
2.7.7.56 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.56 0.001
-
tRNA-C-C-A-C2 pH 8.0, 37°C Escherichia coli
2.7.7.56 0.001
-
tRNA-C-C-A-C3 pH 8.0, 37°C Escherichia coli
2.7.7.56 2
-
phosphate with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.56 Co2+ 30% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM Escherichia coli
2.7.7.56 KCl 50-75 mM stimulates 2fold Escherichia coli
2.7.7.56 Mg2+ maximal at 5-10 mM Escherichia coli
2.7.7.56 Mn2+ 40% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM Escherichia coli
2.7.7.56 additional information no effect: Zn2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.56 45000 50000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.56 tRNAn+1 + phosphate Escherichia coli role in tRNA processing and RNA degradation tRNA + a nucleoside diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.56 Escherichia coli
-
gene rph, orfE
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.56 poly(A) + phosphate
-
Escherichia coli ?
-
r
2.7.7.56 tRNA-C-C-A-C2 + phosphate
-
Escherichia coli ?
-
?
2.7.7.56 tRNA-C-C-A-C3 + phosphate
-
Escherichia coli ?
-
?
2.7.7.56 tRNAn+1 + phosphate substrate specificity Escherichia coli tRNA + a nucleoside diphosphate
-
r
2.7.7.56 tRNAn+1 + phosphate dependent on phosphate Escherichia coli tRNA + a nucleoside diphosphate
-
r
2.7.7.56 tRNAn+1 + phosphate role in tRNA processing and RNA degradation Escherichia coli tRNA + a nucleoside diphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.56 37
-
assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.56 45
-
10 min, fairly stable up to Escherichia coli
2.7.7.56 55
-
10 min, about 50% loss of activity Escherichia coli
2.7.7.56 65
-
10 min, complete inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.56 8 9
-
Escherichia coli