EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.56 | EDTA | - |
Escherichia coli | |
2.7.7.56 | N-ethylmaleimide | - |
Escherichia coli | |
2.7.7.56 | p-hydroxymercuribenzoate | - |
Escherichia coli | |
2.7.7.56 | Zn2+ | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.56 | 0.001 | - |
tRNA-C-C-A-C2 | pH 8.0, 37°C | Escherichia coli | |
2.7.7.56 | 0.001 | - |
tRNA-C-C-A-C3 | pH 8.0, 37°C | Escherichia coli | |
2.7.7.56 | 2 | - |
phosphate | with tRNA-C-C-A-C2 or tRNA-C-C-A-C3, pH 8.0, 37°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.56 | Co2+ | 30% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM | Escherichia coli | |
2.7.7.56 | KCl | 50-75 mM stimulates 2fold | Escherichia coli | |
2.7.7.56 | Mg2+ | maximal at 5-10 mM | Escherichia coli | |
2.7.7.56 | Mn2+ | 40% of the activity with Mg2+, 2 mM somewhat more effective than 5 mM | Escherichia coli | |
2.7.7.56 | additional information | no effect: Zn2+ | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.56 | 45000 | 50000 | gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.56 | tRNAn+1 + phosphate | Escherichia coli | role in tRNA processing and RNA degradation | tRNA + a nucleoside diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.56 | Escherichia coli | - |
gene rph, orfE | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.56 | poly(A) + phosphate | - |
Escherichia coli | ? | - |
r | |
2.7.7.56 | tRNA-C-C-A-C2 + phosphate | - |
Escherichia coli | ? | - |
? | |
2.7.7.56 | tRNA-C-C-A-C3 + phosphate | - |
Escherichia coli | ? | - |
? | |
2.7.7.56 | tRNAn+1 + phosphate | substrate specificity | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
2.7.7.56 | tRNAn+1 + phosphate | dependent on phosphate | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r | |
2.7.7.56 | tRNAn+1 + phosphate | role in tRNA processing and RNA degradation | Escherichia coli | tRNA + a nucleoside diphosphate | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.56 | 37 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.56 | 45 | - |
10 min, fairly stable up to | Escherichia coli |
2.7.7.56 | 55 | - |
10 min, about 50% loss of activity | Escherichia coli |
2.7.7.56 | 65 | - |
10 min, complete inactivation | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.56 | 8 | 9 | - |
Escherichia coli |