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Literature summary extracted from

  • Lehman, I.R.
    T4 DNA polymerase (1974), Methods Enzymol., 29, 46-53.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 4-hydroxymercuribenzoate 95% inhibition at 0.4 mM Tequatrovirus T4
2.7.7.7 Salt optimal activity in presence of total salt concentration of approximately 0.1 M, 97% inhibition at 0.3 M Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Tequatrovirus T4
2.7.7.7 Mg2+ optimal concentration: 6 mM Tequatrovirus T4
2.7.7.7 Mn2+ optimal concentration: 0.1 mM Tequatrovirus T4
2.7.7.7 Mn2+ 25% of the activity with Mg2+ Tequatrovirus T4
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 114000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Tequatrovirus T4
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Tequatrovirus T4
-
 Escherichia coli infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 Escherichia coli infected with Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 1.033
-
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity Tequatrovirus T4 diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 monomer 1 * 114000 Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8 9
-
 Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.7 7.5 9.7 about 50% of activity maximum at pH 7.5 and pH 9.7 Tequatrovirus T4