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Literature summary extracted from

  • Anderson, J.M.; Hori, K.; Cormier, M.J.
    A bioluminescence assay for PAP(3',5'-diphosphoadenosine) and PAPS (3'-phosphoadenylyl sulfate) (1978), Methods Enzymol., 57, 244-257.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.8.2.10 activity is labile, can be stabilized by continous presence of 0.05 ml/l 2-mercaptoethanol Renilla reniformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.10 Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate Renilla reniformis
-
 Renilla luciferin + 3'-phosphoadenylylsulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.10 Renilla reniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.10 partial Renilla reniformis

Storage Stability

EC Number Storage Stability Organism
2.8.2.10 -80°C, requirement of 2-mercaptoethanol in storage buffer, stable for at least several months Renilla reniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.10 benzyl luciferin + 3'-phosphoadenylylsulfate fully active analog of coelenterate-type luciferin, equally effective as substrate Renilla reniformis benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
-
 r
2.8.2.10 benzyl luciferyl sulfate + adenosine 3',5'-bisphosphate
-
 Renilla reniformis benzyl luciferin + 3'-phosphoadenylylsulfate
-
 r
2.8.2.10 Renilla luciferyl sulfate + adenosine 3',5'-bisphosphate
-
 Renilla reniformis Renilla luciferin + 3'-phosphoadenylylsulfate
-
 r