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Literature summary extracted from

  • Francesconi, M.A.; Donella-Deana, A.; Furlanetto, V.; Cavallini, L.; Palatini, P.; Deana, R.
    Further purification and characterization of the succinyl-CoA:3-hydroxy-3-methylglutarate coenzyme A transferase from rat-liver mitochondria (1989), Biochim. Biophys. Acta, 999, 163-170.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.3.13 additional information not activated by 2-mercaptoethanol Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.13 acetate
-
 Rattus norvegicus
2.8.3.13 acetoacetate
-
 Rattus norvegicus
2.8.3.13 acetoacetyl-CoA non-competitive inhibition Rattus norvegicus
2.8.3.13 acetyl-CoA non-competitive inhibition Rattus norvegicus
2.8.3.13 butyryl-CoA non-competitive inhibition Rattus norvegicus
2.8.3.13 carnitine
-
 Rattus norvegicus
2.8.3.13 Cl- high concentration Rattus norvegicus
2.8.3.13 ClO4- high concentration Rattus norvegicus
2.8.3.13 coenzyme A non-competitive inhibition Rattus norvegicus
2.8.3.13 F- high concentration Rattus norvegicus
2.8.3.13 I- high concentration Rattus norvegicus
2.8.3.13 additional information only negligibly affected by sulfhydryl reagents and by the cations Li+, Cd2+, Ca2+, Mg2+, Mn2+ and Fe2+ Rattus norvegicus
2.8.3.13 succinate competitive inhibition of the product Rattus norvegicus
2.8.3.13 ZnCl2
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.13 0.25
-
 succinyl-CoA pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutarate Rattus norvegicus
2.8.3.13 0.32
-
 3-hydroxy-3-methylglutaryl-CoA pH 7.8, 30°C, cosubstrate succinate or malonate, reverse reaction Rattus norvegicus
2.8.3.13 0.73
-
 succinate pH 7.8, 30°C, cosubstrate 3-hydroxy-3-methylglutaryl-CoA, reverse reaction Rattus norvegicus
2.8.3.13 1.78
-
 3-hydroxy-3-methylglutarate pH 7.8, 30°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.13 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.13 48000 52000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.13 succinyl-CoA + 3-hydroxy-3-methylglutarate Rattus norvegicus involved in regulation of the tricarboxylic acid cycle rate and ketogenesis succinate + 3-hydroxy-3-methylglutaryl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.13
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.13 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.13 0.248
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.3.13 -20°C, 0.1 M KH2PO4, pH 7.8, retains 70% of its activity after 2 months of storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.13 succinyl-CoA + 3-hydroxy-3-methylglutarate
-
 Rattus norvegicus succinate + 3-hydroxy-3-methylglutaryl-CoA
-
 r
2.8.3.13 succinyl-CoA + 3-hydroxy-3-methylglutarate involved in regulation of the tricarboxylic acid cycle rate and ketogenesis Rattus norvegicus succinate + 3-hydroxy-3-methylglutaryl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.3.13 tetramer 4 * 12000-14000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.3.13 succinylCoA:3-hydroxy-3-methylglutarate coenzyme A transferase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.13 7.8
-
-
 Rattus norvegicus