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Literature summary extracted from

  • Hansson, A.; Willows, R.D.; Roberts, T.H.; Hansson, M.
    Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase subunit form defective AAA+ hexamers (2002), Proc. Natl. Acad. Sci. USA, 99, 13944-13949.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.6.1.1 D207N altered restriction enzyme site, no measurable magnesium chelatase activity, ATPase activity not affected Hordeum vulgare
6.6.1.1 L111F altered restriction enzyme site, no measurable magnesium chelatase activity, ATPase activity not affected Hordeum vulgare
6.6.1.1 R298K altered restriction enzyme site, no measurable magnesium chelatase activity, ATPase activity not affected Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.6.1.1 0.026
-
 ATP pH 8.5, 32ºC Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Hordeum vulgare
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Hordeum vulgare
-
 barley
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Hordeum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.6.1.1 caryopsis
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Hordeum vulgare ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
6.6.1.1 ? x * 90000-130000, gel filtration, BchI subunit Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.6.1.1 32
-
 assay at Hordeum vulgare