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Literature summary extracted from

  • O'Toole, G.A.; Escalante-Semerena, J.C.
    Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate (1995), J. Biol. Chem., 270, 23560-23569.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.156 GTP stimulates kinase activity Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.156 overexpressed in strain JE3207 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 overexpressed in strain JE3207 Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
2.7.1.156 substrates provides little protection from inhibition by DTNB Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 substrates provides little protection from inhibition by DTNB, GTP provides slight protection from inhibition of the guanylyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.156 DTNB
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 DTNB
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.156 0.066
-
 5'-deoxyadenosylcobinamide pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 0.206
-
 ATP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.0213
-
 GTP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.102
-
 5'-deoxyadenosylcobinamide phosphate pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.156 19700
-
 predicted mass, based on DNA sequence Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 22000
-
 2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 49000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 19700
-
 predicted mass, based on DNA sequence Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 22000
-
 2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 49000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.156 ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
 adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium assembly of the nucleotide loop of cobalamin adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 GTP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium
-
 adenosylcobinamide phosphate + GDP
-
 ?
2.7.1.156 GTP + adenosylcobinamide Salmonella enterica subsp. enterica serovar Typhimurium assembly of the nucleotide loop of cobalamin adenosylcobinamide phosphate + GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate Salmonella enterica subsp. enterica serovar Typhimurium assembly of the nucleotide loop of cobalamin diphosphate + adenosylcobinamide-GDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.1.156 in presence of oxygen kinase activity is lost Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 in presence of oxygen guanylyltransferase activity is lost Salmonella enterica subsp. enterica serovar Typhimurium

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.156
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.156 0.00013
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.000202
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.1.156 4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 4°C, stored under a N2 headspace, retains 98% of its activity for at least 3 weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.156 ATP + 5'-deoxyadenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5'-deoxyadenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 ATP + adenosylcobinamide assembly of the nucleotide loop of cobalamin Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
 ?
2.7.1.156 GTP + adenosylcobinamide
-
 Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + GDP
-
 ?
2.7.1.156 GTP + adenosylcobinamide assembly of the nucleotide loop of cobalamin Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + GDP
-
 ?
2.7.1.156 additional information CobU does not have any detectable ATP:adenosyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 GTP + adenosylcobinamide phosphate assembly of the nucleotide loop of cobalamin Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + adenosylcobinamide-GDP
-
 ?
2.7.7.62 additional information CobU is a bifunctional enzyme that has both kinase EC 27.1.1.156 and guanyltransferase EC 2.7.7.62 activities, ATP does not serve as an efficient substrate for the CobU transferase activity, CobU does not have any detectable ATP:adenosyltransferase activity Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.156 dimer 2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 dimer 2 * 22000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.7.1.156 bifunctional CobU enzyme
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 CobU protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 bifunctional CobU enzyme
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 CobU
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 CobU protein
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.156 37
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 37
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.156 25 60 retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 25 60 retains 50% activity in this range, temperatures above 60°C results in drastic decrease of activity Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.156 0.191
-
 ATP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.156 0.251
-
 5'-deoxyadenosylcobinamide pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.058
-
 GTP pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 0.093
-
 5'-deoxyadenosylcobinamide phosphate pH 8.8, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.156 8.8 9
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 8.8 9
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.156 8.5 9.5 50% activity retains in this range Salmonella enterica subsp. enterica serovar Typhimurium
2.7.7.62 8.5 9.5 50% activity retains in this range Salmonella enterica subsp. enterica serovar Typhimurium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium predicted pI for the denatured protein
-
 5.3
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
 7.2
2.7.1.156 Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
 7.5
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium predicted pI for the denatured protein
-
 5.3
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
 7.2
2.7.7.62 Salmonella enterica subsp. enterica serovar Typhimurium experimetally determined isoelectric point
-
 7.5