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Literature summary extracted from

  • Eger, B.T.; Okamoto, K.; Enroth, C.; Sato, M.; Nishino, T.; Pai, E.F.; Nishino, T.
    Purification, crystallization and preliminary X-ray diffraction studies of xanthine dehydrogenase and xanthine oxidase isolated from bovine milk (2000), Acta Crystallogr. Sect. D, 56, 1656-1658.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.4 batch method, 2.5 and 3.3 A resolution for irreversible proteolytically cleaved xanthine oxidase form, 2.1 A resolution for dehydrogenase form Bos taurus
1.17.3.2 batch method most suitable for crystallization Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.3.2 allopurinol used as anti-gout drug Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron-sulfur-center 2 Fe2-S2-clusters, located at the N-terminal 20 kDa domain Bos taurus
1.17.1.4 molybdenum-center C-terminal 85 kDa molybdopterin-binding domain Bos taurus
1.17.3.2 Iron iron-molybdenum protein Bos taurus
1.17.3.2 Molybdenum an iron-molybdenum protein Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 145000
-
 2 * 145000, data from crystallization Bos taurus
1.17.1.4 290000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O Bos taurus
-
 xanthine + NADH + H+
-
 ?
1.17.1.4 xanthine + NAD+ + H2O Bos taurus
-
 urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Bos taurus
-
-
-
1.17.3.2 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.17.3.2 additional information
-
 Bos taurus
1.17.3.2 proteolytic modification
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4
-
 Bos taurus
1.17.3.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 milk
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O
-
 Bos taurus xanthine + NADH + H+
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Bos taurus urate + NADH
-
 ?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
 Bos taurus urate + 2 H2O2
-
 ?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Bos taurus uric acid + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 dimer 2 * 145000, data from crystallization Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD one FAD per subunit, central 40 kDa FAD-domain Bos taurus
1.17.1.4 additional information one molybdopterin-cofactor, two Fe2-S2-cluster, one FAD per subunit Bos taurus
1.17.1.4 NAD+
-
 Bos taurus
1.17.3.2 FAD flavoprotein Bos taurus