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Literature summary extracted from
- Purification and some properties of xanthine dehydrogenase from wheat leaves (1998), Plant Sci., 134, 89-102.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | Urate | 30% activation of hypoxanthine-NAD+ activity | Triticum aestivum |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | 4-hydroxymercuribenzoate | 76% inhibition of xanthine-NAD+-activity at 0.002 mM | Triticum aestivum | |
| 1.17.1.4 | diethyldithiocarbamate | 72% inhibition of xanthine-NAD+-activity at 10 mM | Triticum aestivum | |
| 1.17.1.4 | NaCN | 69% inhibition of xanthine-NAD+-activity at 3.3 mM | Triticum aestivum | |
| 1.17.1.4 | o-phenanthroline | 12% inhibition of xanthine-NAD+-activity at 7.5 mM | Triticum aestivum | |
| 1.17.1.4 | Salicylhydroxamic acid | 71% inhibition of xanthine-NAD+-activity at 5 mM | Triticum aestivum | |
| 1.17.1.4 | Urate | 15% inhibition of xanthine-NAD+-activity | Triticum aestivum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.4 | 0.0041 | - |
hypoxanthine | electron acceptor: NAD+ | Triticum aestivum | |
| 1.17.1.4 | 0.0056 | - |
phenazine methosulfate | electron donor: xanthine | Triticum aestivum | |
| 1.17.1.4 | 0.0057 | - |
NADH | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
| 1.17.1.4 | 0.0075 | - |
hypoxanthine | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
| 1.17.1.4 | 0.0082 | - |
xanthine | electron acceptor: NAD+ | Triticum aestivum | |
| 1.17.1.4 | 0.0086 | - |
xanthine | electron acceptor: nitroblue tetrazolium | Triticum aestivum | |
| 1.17.1.4 | 0.0113 | - |
nitroblue tetrazolium | electron donor: xanthine | Triticum aestivum | |
| 1.17.1.4 | 0.171 | - |
NAD+ | electron donor: xanthine | Triticum aestivum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 350000 | - |
native PAGE | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | 2-amino-4-hydroxy-pterin + NAD+ + H2O | Triticum aestivum | i.e. pterin | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxy-pterin + NAD+ + H2O | Triticum aestivum | 11% of the activity compared to xanthine | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | purine + NAD+ + H2O | Triticum aestivum | poor substrate | ? + NADH | - |
? | |
| 1.17.1.4 | xanthine + NAD+ + H2O | Triticum aestivum | strict dehydrogenase activity, no utilization of O2 | urate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | Triticum aestivum | - |
wheat | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.4 | to homogeneity, chromatography techniques | Triticum aestivum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | leaf | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 0.23 | - |
xanthine-NAD+ assay | Triticum aestivum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.17.1.4 | 4°C, 25 mM Tris-boric acid, pH 8.5, 2.5 mM EDTA, 2 mM 2-mercaptoethanol, 0.2 mM sodium molybdate, one year | Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | 2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxy-pterin + NAD+ + H2O | 11% of the activity compared to xanthine | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxypterin + NAD+ + H2O | 11% of the activity compared to xanthine | Triticum aestivum | isoxanthopterin + NADH | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | isoxanthopterin + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | 2-amino-4-hydroxypterin + nitroblue tetrazolium + H2O | i.e. pterin | Triticum aestivum | isoxanthopterin + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | 4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | 4-hydroxypyrazolo(3,4-d)pyrimidine + nitroblue tetrazolium + H2O | i.e. allopurinol | Triticum aestivum | 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | acetaldehyde + NAD+ + H2O | very low activity | Triticum aestivum | acetate + NADH + H+ | - |
? | |
| 1.17.1.4 | acetaldehyde + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | acetic acid + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | benzaldehyde + nitroblue tetrazolium + H2O | very low activity | Triticum aestivum | benzoate + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | hypoxanthine + nitroblue tetrazolium | - |
Triticum aestivum | xanthine + ? | - |
? | |
| 1.17.1.4 | NADH + electron acceptor + H2O | electron acceptor: nitroblue tetrazolium | Triticum aestivum | NAD+ + reduced electron acceptor | - |
? | |
| 1.17.1.4 | NADPH + nitroblue tetrazolium + H2O | diaphorase activity | Triticum aestivum | NADP+ + reduced nitroblue tetrazolium | - |
? | |
| 1.17.1.4 | purine + NAD+ + H2O | poor substrate | Triticum aestivum | ? + NADH | - |
? | |
| 1.17.1.4 | purine + nitroblue tetrazolium | low activity | Triticum aestivum | ? | - |
? | |
| 1.17.1.4 | xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Triticum aestivum | urate + ? | - |
? | |
| 1.17.1.4 | xanthine + methyl viologen + H2O | - |
Triticum aestivum | urate + reduced methyl viologen | - |
? | |
| 1.17.1.4 | xanthine + methylene blue + H2O | 3fold higher activity compared to NAD+ as electron acceptor | Triticum aestivum | urate + reduced methylene blue | - |
? | |
| 1.17.1.4 | xanthine + NAD+ + H2O | strict dehydrogenase activity, no utilization of O2 | Triticum aestivum | urate + NADH | - |
? | |
| 1.17.1.4 | xanthine + nitroblue tetrazolium + H2O | - |
Triticum aestivum | urate + ? | - |
? | |
| 1.17.1.4 | xanthine + phenazine methosulfate + H2O | 5fold higher activity compared to NAD+ as electron acceptor | Triticum aestivum | urate + ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 8 | 8.6 | xanthine-NAD+, NADH-nitroblue tetrazolium or xanthine-nitroblue tetrazolium assay | Triticum aestivum |
| 1.17.1.4 | 9.8 | - |
second optimum for NADH-nitroblue tetrazolium assay | Triticum aestivum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | flavin | - |
Triticum aestivum | |
| 1.17.1.4 | additional information | - |
Triticum aestivum | |
| 1.17.1.4 | NAD+ | cannot be replaced by NADP+ | Triticum aestivum | |
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Release 2023.1 (January 2023)
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