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Literature summary extracted from

  • Self, W.T.; Stadtman, T.C.
    Selenium-dependent metabolism of purines: A selenium-dependent purine hydroxylase and xanthine dehydrogenase were purified from Clostridium purinolyticum and characterized (2000), Proc. Natl. Acad. Sci. USA, 97, 7208-7213.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 KCN cyanolyzable selenium, 75% inhibition at 15 mM Gottschalkia purinilytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.0045
-
 xanthine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
1.17.1.4 0.5
-
 hypoxanthine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
1.17.1.4 1
-
 4-hydroxypyrazolo(3,4-d)pyrimidine electron acceptor: 2,6-dichlorophenolindophenol Gottschalkia purinilytica
1.17.1.4 1
-
 4-hydroxypyrazolo(3,4-d)pyrimidine i.e. allopurinol Gottschalkia purinilytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Se
-
 Gottschalkia purinilytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 16000
-
 alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
1.17.1.4 35000
-
 alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
1.17.1.4 80000
-
 alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica
1.17.1.4 529000
-
 gel filtration Gottschalkia purinilytica

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Gottschalkia purinilytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques Gottschalkia purinilytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture
-
 Gottschalkia purinilytica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 2.6
-
 xanthine-2,6-dichlorophenolindophenol assay Gottschalkia purinilytica

Storage Stability

EC Number Storage Stability Organism
1.17.1.4 -70°C, 6 months Gottschalkia purinilytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 1-methylxanthine + ferricyanide + H2O
-
 Gottschalkia purinilytica 1-methylurate + ferrocyanide
-
 ?
1.17.1.4 2-OH-purine + ferricyanide + H2O
-
 Gottschalkia purinilytica ? + ferrocyanide
-
 ?
1.17.1.4 4-hydroxypyrazolo(3,4-d)pyrimidine + ferricyanide + H2O i.e. allopurinol Gottschalkia purinilytica 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + ferrocyanide
-
 ?
1.17.1.4 hypoxanthine + ferricyanide
-
 Gottschalkia purinilytica xanthine + ferrocyanide
-
 ?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Gottschalkia purinilytica urate + ?
-
 ?
1.17.1.4 xanthine + ferricyanide + H2O preferred substrates, does not act with NAD+ or NADP+ Gottschalkia purinilytica urate + ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 dodecamer alpha4, 4 * 80000 + beta4, 4 * 35000 + gamma4, 4 * 16000, SDS-PAGE Gottschalkia purinilytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 ferricyanide does not act with NAD+ or NADP+ Gottschalkia purinilytica
1.17.1.4 additional information
-
 Gottschalkia purinilytica