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Literature summary extracted from

  • Sin, I.L.
    Purification and properties of xanthine dehydrogenase from Pseudomonas acidovorans (1975), Biochim. Biophys. Acta, 410, 12-20.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.17.1.4 crude and purified enzyme preparations form readily insoluble inactive aggregates, not stable to freezing and thawing Delftia acidovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 adenine some non-competitive inhibition Delftia acidovorans
1.17.1.4 guanine some inhibition Delftia acidovorans
1.17.1.4 purine some inhibition Delftia acidovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.07
-
 xanthine NAD+ Delftia acidovorans
1.17.1.4 0.07
-
 xanthine cosubstrate Delftia acidovorans
1.17.1.4 0.12
-
 NAD+ cosubstrate: xanthine Delftia acidovorans
1.17.1.4 0.16
-
 NAD+ cosubstrate: hypoxanthine Delftia acidovorans
1.17.1.4 0.29
-
 hypoxanthine cosubstrate: NAD+ Delftia acidovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 63000
-
 2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans
1.17.1.4 81000
-
 2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans
1.17.1.4 275000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O Delftia acidovorans
-
 xanthine + NADH + H+
-
 ?
1.17.1.4 purine + NAD+ + H2O Delftia acidovorans poor substrate ? + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O Delftia acidovorans
-
 urate + NADH
-
 ?
1.17.1.4 xanthine + NADP+ + H2O Delftia acidovorans 11% of the activity compared to NAD+ urate + NADPH
-
 ?
1.17.1.4 xanthine + O2 + H2O Delftia acidovorans low activity urate + O2- + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Delftia acidovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 near homogeneity, chromatography techniques Delftia acidovorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture
-
 Delftia acidovorans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O
-
 Delftia acidovorans xanthine + NADH + H+
-
 ?
1.17.1.4 NADH + electron acceptor + H2O extremely slow reoxidation rate Delftia acidovorans NAD+ + reduced electron acceptor
-
 ?
1.17.1.4 purine + NAD+ + H2O poor substrate Delftia acidovorans ? + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Delftia acidovorans urate + NADH
-
 ?
1.17.1.4 xanthine + NADP+ + H2O 11% of the activity compared to NAD+ Delftia acidovorans urate + NADPH
-
 ?
1.17.1.4 xanthine + O2 + H2O low activity Delftia acidovorans urate + O2- + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 tetramer 2 * 81000 + 2 * 63000, SDS-PAGE Delftia acidovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 7.9
-
-
 Delftia acidovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Delftia acidovorans
1.17.1.4 NADP+ 11% of the activity compared to NAD+ Delftia acidovorans