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Literature summary extracted from

  • Xiang, Q.; Edmondson, D.E.
    Purification and characterization of a prokaryotic xanthine dehydrogenase from Comamonas acidovorans (1996), Biochemistry, 35, 5441-5450.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 4-hydroxypyrazolo(3,4-d)pyrimidine i.e. allopurinol; rapid inactivation under anaerobic conditions at 0.1 mM Delftia acidovorans
1.17.1.4 KCN irreversible inactivation Delftia acidovorans
1.17.1.4 Sodium dithionite reduction of enzyme Delftia acidovorans
1.17.1.4 xanthine reduction of enzyme Delftia acidovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.066
-
 xanthine electron acceptor: NAD+ Delftia acidovorans
1.17.1.4 0.16
-
 NAD+ electron donor: xanthine Delftia acidovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Iron-sulfur-center 2 Fe2-S2-clusters Delftia acidovorans
1.17.1.4 molybdenum-center contains molybdopterin mononucleotide rather than molybdopterin dinucleotide Delftia acidovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 60000
-
 alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans
1.17.1.4 90000
-
 alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans
1.17.1.4 287000
-
 gel filtration Delftia acidovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O Delftia acidovorans
-
 urate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Delftia acidovorans
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.17.1.4 phosphoprotein 3.6 mol per alpha/beta-protomer Delftia acidovorans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography steps Delftia acidovorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture
-
 Delftia acidovorans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 50
-
-
 Delftia acidovorans

Storage Stability

EC Number Storage Stability Organism
1.17.1.4 -80°C, 6 months Delftia acidovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 NADH + electron acceptor + H2O NADH diaphorase activity with several acceptors Delftia acidovorans NAD+ + reduced electron acceptor
-
 ?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Delftia acidovorans urate + ?
-
 ?
1.17.1.4 xanthine + ferricyanide + H2O
-
 Delftia acidovorans urate + ferrocyanide
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Delftia acidovorans urate + NADH
-
 ?
1.17.1.4 xanthine + nitroblue tetrazolium + H2O
-
 Delftia acidovorans urate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE Delftia acidovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.4 120
-
 xanthine electron acceptor: NAD+ Delftia acidovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD
-
 Delftia acidovorans
1.17.1.4 additional information molybdoironflavoprotein: ratio of iron to FAD to molybdenum is 4 : 1 : 1 Delftia acidovorans
1.17.1.4 NAD+ physiological cofactor Delftia acidovorans