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Literature summary extracted from

  • Wagner, R.; Cammack, R.; Andreesen, J.R.
    Purification and characterization of xanthine dehydrogenase from Clostridium acidiurici grown in the presence of selenium (1984), Biochim. Biophys. Acta, 791, 63-74.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.17.1.4 complete loss of activity when stored below 0°C Gottschalkia acidurici

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.44
-
 methyl viologen electron donor: xanthine Gottschalkia acidurici
1.17.1.4 1.35
-
 xanthine electron acceptor: methyl viologen Gottschalkia acidurici

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Fe non-heme iron-sulfur groups, 7.7 g-atom Fe, 7.5 g- atom S2- per mol enzyme Gottschalkia acidurici
1.17.1.4 Iron-sulfur-center 2 Fe2-S2-clusters Gottschalkia acidurici
1.17.1.4 Mo
-
 Gottschalkia acidurici
1.17.1.4 molybdenum-center 1.8 g-atom molybdenum per native molecule Gottschalkia acidurici
1.17.1.4 Se 0.13 g-atom selenium per native enzyme molecule Gottschalkia acidurici
1.17.1.4 Tungsten 0.12 g-atom tungsten per native enzyme molecule Gottschalkia acidurici

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 26000
-
 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
1.17.1.4 53000
-
 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
1.17.1.4 56000
-
 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
1.17.1.4 83000
-
 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
1.17.1.4 110000
-
 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici
1.17.1.4 224000
-
 gel filtration Gottschalkia acidurici

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.4 xanthine + O2 + H2O Gottschalkia acidurici very low activity urate + O2- + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Gottschalkia acidurici
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4 to homogeneity, chromatography techniques, preparative gel electrophoresis Gottschalkia acidurici

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 cell culture selenium is needed for xanthine dehydrogenase formation Gottschalkia acidurici
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 385
-
-
 Gottschalkia acidurici

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O i.e. allopurinol Gottschalkia acidurici 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen
-
 ?
1.17.1.4 4-hydroxypyrazolo(3,4-d)pyrimidine + methyl viologen + H2O 8% of the activity compared to xanthine Gottschalkia acidurici 4,6-dihydroxypyrazolo(3,4-d)pyrimidine + reduced methyl viologen
-
 ?
1.17.1.4 5-aminoimidazol-4-carboxamide + methyl viologen + H2O 1.1% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
 ?
1.17.1.4 6-mercaptopurine + methyl viologen + H2O 9.5% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
 ?
1.17.1.4 hypoxanthine + methyl viologen + H2O 7% of the activity compared to xanthine Gottschalkia acidurici xanthine + reduced methyl viologen
-
 ?
1.17.1.4 purine + methyl viologen + H2O 2% of the activity compared to xanthine Gottschalkia acidurici ? + reduced methyl viologen
-
 ?
1.17.1.4 xanthine + 2,6-dichlorophenolindophenol + H2O 2.5% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ?
-
 ?
1.17.1.4 xanthine + benzyl viologen + H2O 52% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + reduced benzyl viologen
-
 ?
1.17.1.4 xanthine + ferricyanide + H2O 99% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ferrocyanide
-
 ?
1.17.1.4 xanthine + methyl viologen + H2O best substrates tested, no activity with NAD+ or NADP+, 40% of the activity in the reverse reaction Gottschalkia acidurici urate + reduced methyl viologen
-
 r
1.17.1.4 xanthine + methylene blue + H2O 87% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + reduced methylene blue
-
 ?
1.17.1.4 xanthine + nitroblue tetrazolium + H2O 32% of the activity compared to methyl viologen as electron acceptor Gottschalkia acidurici urate + ?
-
 ?
1.17.1.4 xanthine + O2 + H2O very low activity Gottschalkia acidurici urate + O2- + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 pentamer 1 * 110000 + 1 * 83000 + 1 * 56000 + 1 * 53000 + 1 * 26000, SDS-PAGE Gottschalkia acidurici

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.4 55
-
-
 Gottschalkia acidurici

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 7
-
 optimum value in the reverse reaction with urate and reduced methyl viologen Gottschalkia acidurici
1.17.1.4 8.5
-
 optimum value for oxidation of xanthine and reduction of methyl viologen Gottschalkia acidurici

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 FAD 1.69 mol FAD per mol enzyme Gottschalkia acidurici
1.17.1.4 additional information molybdoironflavoprotein: ratio of non-heme iron to acid-labile sulfur to FAD to molybdenum to tungsten to selenium is 7.7 : 7.5 : 1.7 : 1.8 : 0.12 : 0.13 Gottschalkia acidurici