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Literature summary extracted from
- Kinetics and stability of immobilized chicken liver xanthine dehydrogenase (1979), Biotechnol. Bioeng., 21, 1767-1786.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.17.1.4 | synthesis | immobilized xanthine dehydrogenase for use in organic synthesis | Gallus gallus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.4 | 0.017 | - |
xanthine | pH 7.9, increasing Km with increasing pH | Gallus gallus |  |
| 1.17.1.4 | 0.019 | - |
NAD+ | pH 7.9, pH dependence, increasing Km with increasing pH | Gallus gallus | |
| 1.17.1.4 | 0.035 | - |
xanthine | immobilized enzyme preparation, pH 7.9, pH dependence, minimum Km at pH 8.1, increasing values below and above | Gallus gallus | |
| 1.17.1.4 | 0.054 | - |
NAD+ | immobilized enzyme preparation, pH 7.9, pH dependence, minimum Km at pH 8.1, increasing values below and above | Gallus gallus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | xanthine + NAD+ + H2O | Gallus gallus | - |
urate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | Gallus gallus | - |
chicken | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.4 | immobilized on n-octylamine-substituted Sepharose 4B | Gallus gallus |
| 1.17.1.4 | to homogeneity, chromatography techniques | Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.17.1.4 | liver | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.4 | xanthine + NAD+ + H2O | - |
Gallus gallus | urate + NADH | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 30 | - |
- |
Gallus gallus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.4 | 4 | - |
free enzyme, half-life: 18 days, immobilized enzyme, half-life: 88 days | Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.4 | NAD+ | - |
Gallus gallus | |
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