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Literature summary extracted from

  • Roy, R.; Menon, A.L.; Adams, M.W.W.
    Aldehyde oxidoreductases from Pyrococcus furiosus (2001), Methods Enzymol., 331, 132-144.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.5 Sulfide activation of formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Sulfide 4-5fold increase in activity at room temperature and pH 8.0 at 20 mM, in presence of 20 mM dithionite Pyrococcus furiosus
1.2.7.5 Tungsten stimulates cell growth Pyrococcus furiosus
1.2.7.5 Tungsten stimulates cell growth Thermococcus litoralis
1.2.7.6 Tungsten stimulates cell growth when added to the medium Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.7.5
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.B2 0.8
-
 glutaric dialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 8
-
 Succinic semialdehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 15
-
 phenylpropionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 25
-
 formaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 25
-
 Indole-3-acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 60
-
 acetaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.B2 62
-
 propionaldehyde 80°C, pH 8.4 Pyrococcus furiosus
1.2.7.5 0.016
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.028
-
 isovalerylaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.05
-
 Indoleacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.057
-
 benzaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.076
-
 phenylacetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.14
-
 crotonaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.15
-
 propionaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 0.8
-
 glutaric dialdehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 1.42
-
 formaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Thermococcus sp.
1.2.7.5 8
-
 Succinic semialdehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 15
-
 phenylpropionaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 25
-
 formaldehyde formaldehyde ferredoxin oxidoreductase, pH 8.4, 80°C, with benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 25
-
 Indoleacetaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 60
-
 acetaldehyde pH 8.4, 85°C, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.5 60
-
 propionaldehyde pH 8.4, 80°C, formaldehyde ferredoxin oxidoreductase, benzyl viologen as electron acceptor Pyrococcus furiosus
1.2.7.6 0.028
-
 D-Glyceraldehyde-3-phosphate with ferredoxin as electron acceptor, pH 8.4, 70°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.5 Ca2+ 1 Ca2+ per subunit, formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus furiosus
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus sp.
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Thermococcus litoralis
1.2.7.5 Fe2+ contains 1 iron-sulfur cluster [4Fe-4S] per subunit Pyrococcus endeavori
1.2.7.5 Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
1.2.7.5 Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
1.2.7.5 Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
1.2.7.5 Fe2+ in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori
1.2.7.5 Fe2+ contains 1 additional Fe2+ besides the [4Fe-4S]-cluster Pyrococcus furiosus
1.2.7.5 Mg2+ both enzyme forms Pyrococcus furiosus
1.2.7.5 Mg2+ contains 1 Mg-atom per subunit Pyrococcus furiosus
1.2.7.5 Mg2+ contains 1 Mg-atom per subunit Thermococcus sp.
1.2.7.5 Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
1.2.7.5 Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
1.2.7.5 Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
1.2.7.5 Tungsten in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori
1.2.7.5 Tungsten 1 tungsten per subunit Pyrococcus furiosus
1.2.7.5 Tungsten 1 tungsten per subunit Thermococcus sp.
1.2.7.5 Tungsten 1 tungsten per subunit Thermococcus litoralis
1.2.7.6 Fe2+ 1 [4Fe-4S]2+ cluster per enzyme molecule Pyrococcus furiosus
1.2.7.6 Fe2+ 5-6 iron g-atoms per mol of enzyme Pyrococcus furiosus
1.2.7.6 Mg2+ contains 1 molecule per enzyme Pyrococcus furiosus
1.2.7.6 Tungsten 1 tungsten center per molecule of enzyme Pyrococcus furiosus
1.2.7.6 Zn2+ contains 2 molecules per enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.5 67000
-
 2 * 67000, SDS-PAGE Thermococcus sp.
1.2.7.5 67000
-
 2 * 67000 Pyrococcus furiosus
1.2.7.5 136100
-
-
 Pyrococcus furiosus
1.2.7.5 280000
-
-
 Pyrococcus furiosus
1.2.7.5 280000
-
-
 Thermococcus litoralis
1.2.7.6 73000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Thermococcus sp.
-
 an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Pyrococcus endeavori
-
 an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Pyrococcus furiosus formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Thermococcus litoralis formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin Thermococcus sp. ES1
-
 an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin Pyrococcus furiosus
-
 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.B2 Pyrococcus furiosus O93738
-
-
1.2.7.5 Pyrococcus endeavori
-
 hyperthermophilic archaeon
-
1.2.7.5 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
1.2.7.5 Pyrococcus furiosus
-
 strain DSM 3638
-
1.2.7.5 Pyrococcus furiosus
-
 contains a formaldehyde and an aldehyde ferredoxin oxidoreductase
-
1.2.7.5 Pyrococcus furiosus Q51739 hyperthermophilic archaeon
-
1.2.7.5 Pyrococcus furiosus Q51739 strain DSM 3638
-
1.2.7.5 Pyrococcus furiosus Q51739 gene aor
-
1.2.7.5 Thermococcus litoralis
-
 hyperthermophilic archaeon
-
1.2.7.5 Thermococcus litoralis
-
 formaldehyde ferredoxin oxidoreductase
-
1.2.7.5 Thermococcus sp.
-
 hyperthermophilic archaeon
-
1.2.7.5 Thermococcus sp. ES1
-
 hyperthermophilic archaeon
-
1.2.7.6 Pyrococcus furiosus
-
 hyperthermophilic archaeon
-
1.2.7.6 Pyrococcus furiosus
-
 strain DSM 3638
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.5 aldehyde ferredoxin oxidoreductase, t1/2: 30 min at 23 °C Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase, t1/2: 9 h at 23°C Pyrococcus furiosus
1.2.7.6 O2-sensitive, t1/2 at 23°C: 6 h Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.5 formaldehyde and aldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required Pyrococcus furiosus
1.2.7.5 formaldehyde ferredoxin oxidoreductase, highly reducing and strict anaerobic conditions are required Thermococcus litoralis
1.2.7.6 to homogeneity Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.6 additional information
-
 strictly anaerobic assay conditions Pyrococcus furiosus
1.2.7.6 25
-
 purified enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.B2 acetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus acetate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 formaldehyde + H2O + 2 oxidized ferredoxin
-
 Pyrococcus furiosus formate + 2 H+ + 2 reduced ferredoxin
-
 ?
1.2.7.B2 formaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 glutaric dialdehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus ? + reduced benzyl viologen + H+
-
 ?
1.2.7.B2 indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus indole-3-acetate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 additional information no activity with crotonaldehyde, benzaldehyde, isovalerylaldehyde or phenylacetaldehyde Pyrococcus furiosus ?
-
 ?
1.2.7.B2 phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus phenylpropionate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 propionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus propionate + H+ + reduced benzyl viologen
-
 ?
1.2.7.B2 succinic semialdehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus succinate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 acetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus litoralis an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus endeavori an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Pyrococcus endeavori an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea Pyrococcus furiosus an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin formaldehyde ferredoxin oxidoreductase, enzyme plays a role in peptide fermentation in hyperthermophilic archaea Thermococcus litoralis an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. ES1 an acid + H+ + reduced ferredoxin
-
 ?
1.2.7.5 an aldehyde + H2O + oxidized ferredoxin
-
 Thermococcus sp. ES1 an acid + H+ + reduced ferredoxin
-
 ir
1.2.7.5 benzaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. benzoic acid + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 benzaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis benzoic acid + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus crotonate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. crotonate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis crotonate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 crotonate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. ES1 formate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 glutaraldehyde + H2O + oxidized methyl viologen
-
 Pyrococcus furiosus glutarate + H+ + reduced methyl viologen
-
 ?
1.2.7.5 glutaric dialdehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis ?
-
 ir
1.2.7.5 indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus indoleacetate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 indoleacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. indoleacetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 indoleacetaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis indoleacetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 isovalerylaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. isovalerate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Pyrococcus furiosus isovalerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 isovalerylaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase, no activity Thermococcus litoralis isovalerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 phenylacetaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. phenylacetate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 phenylacetaldehyde + H2O + oxidized benzyl viologen no activity Thermococcus litoralis phenylacetate + H+ + reduced benzyl viologen
-
 ?
1.2.7.5 phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus phenylpropanoate + H+ + reduced benzyl viologen
-
 r
1.2.7.5 phenylpropionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis phenylpropanoate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 propionaldehyde + H2O + oxidized benzyl viologen
-
 Thermococcus sp. propionate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus propionate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 propionaldehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis propionate + H+ + reduced benzyl viologen
-
 ir
1.2.7.5 succinic semialdehyde + H2O + oxidized benzyl viologen
-
 Pyrococcus furiosus succinic acid + H+ + reduced benzyl viologen
-
 r
1.2.7.5 succinic semialdehyde + H2O + oxidized benzyl viologen formaldehyde ferredoxin oxidoreductase Thermococcus litoralis succinic acid + H+ + reduced benzyl viologen
-
 ir
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized benzyl viologen artificial electron acceptor Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced benzyl viologen
-
 ?
1.2.7.6 D-glyceraldehyde-3-phosphate + H2O + oxidized ferredoxin
-
 Pyrococcus furiosus 3-phospho-D-glycerate + H+ + reduced ferredoxin
-
 ?
1.2.7.6 additional information no activity with NAD(P)+ as electron acceptor Pyrococcus furiosus ?
-
 ?
1.2.7.6 additional information no activity with formaldehyde, propionaldehyde, phenylacetaldehyde, butyraldehyde, crotonaldehyde, acetaldehyde, dihydroxyacetone phosphate, glyceraldehyde, benzaldehyde, glucose, glucose 6-phosphate, or glyoxylate Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.5 dimer
-
 Pyrococcus furiosus
1.2.7.5 dimer 2 * 67000, SDS-PAGE Thermococcus sp.
1.2.7.5 dimer 2 * 67000 Pyrococcus furiosus
1.2.7.6 monomer 1 * 73000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.2.7.5 More formaldehyde ferredoxin oxidoreductase and aldehyde ferredoxin oxidoreductase are 2 separate enzymes Pyrococcus furiosus
1.2.7.6 glyceraldehyde-3-phosphate ferredoxin oxidoreductase
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.5 80
-
 assay at Pyrococcus furiosus
1.2.7.5 80
-
 assay at Thermococcus sp.
1.2.7.5 80
-
 assay at Thermococcus litoralis
1.2.7.5 80
-
 assay at Pyrococcus endeavori
1.2.7.6 70
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.5 80
-
 formaldehyde ferredoxin oxidoreductase, t1/2: 6 h Pyrococcus furiosus
1.2.7.5 80
-
 aldehyde ferredoxin oxidoreductase, t1/2: 15 min Pyrococcus furiosus
1.2.7.6 80
-
 t1/2: 15 min Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.5 8.4
-
 assay at Pyrococcus furiosus
1.2.7.5 8.4
-
 assay at Thermococcus sp.
1.2.7.5 8.4
-
 assay at Thermococcus litoralis
1.2.7.5 8.4
-
 assay at Pyrococcus endeavori
1.2.7.6 8.4
-
 assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.5 Ferredoxin
-
 Pyrococcus furiosus
1.2.7.5 Ferredoxin
-
 Thermococcus sp.
1.2.7.5 Ferredoxin
-
 Thermococcus litoralis
1.2.7.5 Ferredoxin
-
 Pyrococcus endeavori
1.2.7.5 tungsten cofactor formaldehyde ferredoxin oxidoreductase Pyrococcus furiosus
1.2.7.5 tungsten cofactor formaldehyde ferredoxin oxidoreductase Thermococcus litoralis
1.2.7.5 tungsten cofactor pterin ring system Pyrococcus furiosus
1.2.7.5 tungsten cofactor pterin ring system Thermococcus sp.
1.2.7.5 tungsten cofactor pterin ring system Thermococcus litoralis
1.2.7.5 tungsten cofactor pterin ring system Pyrococcus endeavori
1.2.7.5 tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus furiosus
1.2.7.5 tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus sp.
1.2.7.5 tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Thermococcus litoralis
1.2.7.5 tungsten-molybdopterin in each subunit tungsten is coordinated by 4 dithiolene sulfur atoms from 2 pterin molecules, together with a single [4Fe-4S]-cluster coordinated by 4 sulfur atoms from 4 cysteine residues Pyrococcus endeavori
1.2.7.6 Ferredoxin
-
 Pyrococcus furiosus
1.2.7.6 tungsten-molybdopterin tungstopterin center Pyrococcus furiosus
1.2.7.6 tungsten-molybdopterin structure Pyrococcus furiosus