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Literature summary extracted from

  • Lu, W.P.; Harder, S.R.; Ragsdale, S.W.
    Controlled potential enzymology of methyl transfer reactions involved in acetyl-CoA synthesis by CO dehydrogenase and the corrinoid/iron-sulfur protein from Clostridium thermoaceticum (1990), J. Biol. Chem., 265, 3124-3133.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.169 Ferredoxin stimulation of acetyl-CoA synthesis from methyl iodide, maximum at 1 nmol ferredoxin/15 nmol of enzyme Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.169 CoA at concentration above 10 mM, 50% inhibition of acetyl-CoA synthesis from methyl iodide at 15 mM Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.169 4.3
-
 CoA pH 7.3, 22°C, acetyl-CoA synthesis from methyl iodide Moorella thermoacetica
2.3.1.169 14.7
-
 CH3I pH 7.3, 22°C Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Fe corrinoid/iron-sulfur protein required Moorella thermoacetica
2.3.1.169 additional information a nucleophilic metal center on enzyme is the active site which accepts the methyl group from the methylated corrinoid/iron-sulfur protein Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.169 additional information Moorella thermoacetica key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.169 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.169
-
 Moorella thermoacetica

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] pathway Moorella thermoacetica
a
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] Enzyme accepts the methyl group from the methylated corrinoid/iron-sulfur protein, binds a carbonyl group from CO, CO2, or the carboxyl of pyruvate, and binds coenzyme A. Then the enzyme catalyses the synthesis of acetyl-CoA from these enzyme bound groups. Additionally the enzyme catalyses two exchange reactions between the methylated corrinoid/iron-sulfur protein and methylated enzyme and between methylated enzyme and the methyl moiety of acetyl-CoA. Moorella thermoacetica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.169 CH3-tetrahydrofolate + CO + HS-CoA
-
 Moorella thermoacetica CH3-CO-S-CoA + tetrahydrofolate
-
 ?
2.3.1.169 CH3I + CO + HS-CoA
-
 Moorella thermoacetica CH3-CO-S-CoA + HI
-
 ?
2.3.1.169 CO + methyl-X + HS-CoA
-
 Moorella thermoacetica CH3-CO-S-CoA + HX
-
 ?
2.3.1.169 additional information key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway Moorella thermoacetica ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.169 5.8
-
 Tris-maleate buffer, the rate of acetyl-CoA synthesis increases with decreasing pH, at pH values below 5.8 the rate of acetyl-CoA synthesis decreases slightly Moorella thermoacetica