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Literature summary extracted from

  • Tan, X.S.; Sewell, C.; Lindahl, P.A.
    Stopped-flow kinetics of methyl group transfer between the corrinoid-iron-sulfur protein and acetyl-Coenzyme A synthase from Clostridium thermoaceticum (2002), J. Am. Chem. Soc., 124, 6277-6284.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.169 CO inhibits the methyl group transfer reaction and synthesis of acetyl-CoA Moorella thermoacetica
2.3.1.169 Dithionite inhibits reverse methyl group transfer, when it is preincubated with methylated enzyme but not when it is preincubated with Co+-iron-sulfur protein Moorella thermoacetica
2.3.1.169 Ti3+-citrate inhibits reverse methyl group transfer, when it is preincubated with methylated enzyme but not when it is preincubated with Co+-iron-sulfur protein Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.169 Fe corrinoid/iron-sulfur protein required Moorella thermoacetica
2.3.1.169 Ni enzyme contains nickel in the A-cluster of the enzyme Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.169 additional information Moorella thermoacetica key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.169 Moorella thermoacetica
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-
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Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] pathway Moorella thermoacetica
a
2.3.1.169 acetyl-CoA + a [Co(I) corrinoid Fe-S protein] = CO + CoA + a [methyl-Co(III) corrinoid Fe-S protein] kinetics of methyl group transfer between the cobalt of the corrinoid/iron-sulfur protein and the nickel of Ni-X-Fe4S4 cluster, called the A-cluster of enzyme, the reaction is reversible Moorella thermoacetica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.169 CO + H2O the multienzyme complex catalyses the reversible oxidation of CO to CO2 Moorella thermoacetica CO2 + H+ + electron
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 r
2.3.1.169 additional information key enzyme in the autotrophic acetyl-CoA pathway, i.e. Wood pathway, enzyme catalyses the final steps in this pathway Moorella thermoacetica ?
-
 ?