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Literature summary extracted from

  • Kittendorf, J.D.; Barcomb, L.M.; Nonekowski, S.T.; Garcia, G.A.
    tRNA-guanine transglycosylase from Escherichia coli: Molecular mechanism and role of aspartate 89 (2001), Biochemistry, 40, 14123-14133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.29 overexpression of D89E, D89D, D89C and D89A in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.29 D89A less than 1% of the activity of the histidine-tagged wild-type enzyme Escherichia coli
2.4.2.29 D89C less than 1% of the activity of the histidine-tagged wild-type enzyme Escherichia coli
2.4.2.29 D89E about 50% of the activity of the histidine-tagged wild-type enzyme Escherichia coli
2.4.2.29 D89N less than 1% of the activity of the histidine-tagged wild-type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.29 0.0001
-
 guanine histidine-tagged wild-type enzyme, pH 7.3 Escherichia coli
2.4.2.29 0.00011
-
 tRNA histidine-tagged mutant enzyme D89E, pH 7.3 Escherichia coli
2.4.2.29 0.00012
-
 tRNA histidine-tagged wild-type enzyme, pH 7.3 Escherichia coli
2.4.2.29 0.00039
-
 tRNA histidine-tagged wild-type enzyme, pH 8.5 Escherichia coli
2.4.2.29 0.00051
-
 tRNA histidine-tagged mutant enzyme D89E, pH 8.5 Escherichia coli
2.4.2.29 0.00057
-
 guanine histidine-tagged wild-type enzyme, pH 8.5 Escherichia coli
2.4.2.29 0.00261
-
 guanine histidine-tagged mutant enzyme D89E, pH 7.3 Escherichia coli
2.4.2.29 0.00632
-
 guanine histidine-tagged mutant enzyme D89E, pH 8.5 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.29 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.29 wild-type and mutant enzymes D89E, D89D, D89C and D89A Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.29 0.59
-
 guanine histidine-tagged mutant enzyme D89E, pH 7.3 Escherichia coli
2.4.2.29 0.71
-
 tRNA histidine-tagged mutant enzyme D89E, pH 7.3 Escherichia coli
2.4.2.29 0.75
-
 guanine histidine-tagged mutant enzyme D89E, pH 8.5 Escherichia coli
2.4.2.29 0.93
-
 tRNA histidine-tagged mutant enzyme D89E, pH 8.5 Escherichia coli
2.4.2.29 1.21
-
 guanine histidine-tagged wild-type enzyme, pH 7.3 Escherichia coli
2.4.2.29 1.21
-
 tRNA histidine-tagged wild-type enzyme, pH 7.3 Escherichia coli
2.4.2.29 5.85
-
 tRNA histidine-tagged wild-type enzyme, pH 8.5 Escherichia coli
2.4.2.29 7.57
-
 guanine histidine-tagged wild-type enzyme, pH 8.5 Escherichia coli