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Literature summary extracted from

  • Zolli, M.; Kobric, D.J.; Brown, E.D.
    Reduction precedes cytidylyl transfer without substrate channeling in distinct active sites of the bifunctional CDP-ribitol synthase from Haemophilus influenzae (2001), Biochemistry, 40, 5041-5048.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.405 expression in Escherichia coli Haemophilus influenzae
2.7.7.40
-
 Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.405 K386A mutant impaired for reduction of D-ribulose. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis Haemophilus influenzae
1.1.1.405 R18A mutant impaired for cytidylyl transfer. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis Haemophilus influenzae
2.7.7.40 K368A kinetic values similar to wild-type enzyme Haemophilus influenzae
2.7.7.40 R18A reduced enzyme activity Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.405 0.017
-
 NADPH mutant R18A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.038
-
 (2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.039
-
 NADPH mutant K386A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.044
-
 NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.076
-
 D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.19
-
 D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.27
-
 D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.6
-
 NADH wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 1.1
-
 D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 2.2
-
 D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 3
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 3.5
-
 D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
2.7.7.40 0.092
-
 CTP pH 7.2, 25°C, K386A mutant Haemophilus influenzae
2.7.7.40 0.099
-
 CTP pH 7.2, 25°C Haemophilus influenzae
2.7.7.40 0.12
-
 D-ribitol 5-phosphate pH 7.2, 25°C, K386A mutant Haemophilus influenzae
2.7.7.40 0.13
-
 D-ribitol 5-phosphate pH 7.2, 25°C Haemophilus influenzae
2.7.7.40 0.39
-
 CTP pH 7.2, 25°C, R18A mutant Haemophilus influenzae
2.7.7.40 1
-
 D-ribitol 5-phosphate pH 7.2, 25°C, R18A mutant Haemophilus influenzae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.40 CTP + D-ribitol 5-phosphate Haemophilus influenzae
-
 diphosphate + CDPribitol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.405 Haemophilus influenzae Q48230 additionally catalyzes the reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40
-
2.7.7.40 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.40
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.405 D-arabinose 5-phosphate + NADPH + H+
-
 Haemophilus influenzae ? + NADP+
-
 ?
1.1.1.405 D-erythrose 4-phosphate + NADPH + H+
-
 Haemophilus influenzae ? + NADP+
-
 ?
1.1.1.405 D-ribose 5-phosphate + NADPH + H+
-
 Haemophilus influenzae ? + NADP+
-
 ?
1.1.1.405 D-ribulose + NADPH + H+
-
 Haemophilus influenzae D-ribitol + NADP+
-
 ?
1.1.1.405 D-ribulose 5-phosphate + NADPH + H+
-
 Haemophilus influenzae D-ribitol 5-phosphate + NADP+
-
 ?
1.1.1.405 D-xylulose 5-phosphate + NADH + H+
-
 Haemophilus influenzae ? + NAD+
-
 ?
1.1.1.405 additional information the synthase first catalyzes the reduction of D-ribulose 5-phosphate followed by cytidylyl transfer to D-ribitol 5-phosphate, with a 650fold kinetic preference for cytidylyl transfer to D-ribitol 5-phosphate over D-ribulose 5-phosphate Haemophilus influenzae ?
-
 ?
2.7.7.40 CTP + D-ribitol 5-phosphate
-
 Haemophilus influenzae diphosphate + CDPribitol
-
 ?
2.7.7.40 additional information enzyme has also ribitol 5-phosphate dehydrogenase activity Haemophilus influenzae ?
-
 ?
2.7.7.40 additional information no activity with ATP, GTP, UTP, ADP or CDP Haemophilus influenzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.405 Bcs1
-
 Haemophilus influenzae
1.1.1.405 bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase
-
 Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.405 0.32
-
 D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.5
-
 (2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 2.3
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 3.6
-
 D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 3.6
-
 D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 4.1
-
 D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 6.8
-
 D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 22
-
 NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 22
-
 D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
2.7.7.40 0.14
-
 D-ribitol 5-phosphate pH 7.2, 25°C, R18A mutant Haemophilus influenzae
2.7.7.40 9.7
-
 D-ribitol 5-phosphate pH 7.2, 25°C, K386A mutant Haemophilus influenzae
2.7.7.40 13
-
 D-ribitol 5-phosphate pH 7.2, 25°C, wild-type enzyme Haemophilus influenzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.405 0.00008
-
 D-arabinose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0001
-
 D-xylulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0002
-
 D-ribose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0002
-
 D-ribulose 5-phosphate mutant K386A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0003
-
 D-ribulose wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0008
-
 NADPH mutant K386A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0013
-
 (2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.0025
-
 D-ribulose 5-phosphate mutant R18A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.029
-
 D-ribulose 5-phosphate wild-type, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.04
-
 NADPH mutant R18A, pH 7.2, 25°C Haemophilus influenzae
1.1.1.405 0.05
-
 NADPH wild-type, pH 7.2, 25°C Haemophilus influenzae