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Literature summary extracted from

  • Roberts, R.M.
    The formation of uridine diphosphate-glucuronic acid in plants. Uridine diphosphate-glucuronic acid pyrophosphorylase from barley seedlings (1971), J. Biol. Chem., 246, 4995-5002.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.44 Mg2+ best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory Hordeum vulgare
2.7.7.44 Mn2+ 53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.44 0.33
-
 D-glucuronic acid 1-phosphate pH 8.0, 30°C Hordeum vulgare
2.7.7.44 0.5
-
 UDP-D-glucuronic acid pH 8.0, 30°C Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.44 Ca2+ 36% of the activation with Mg2+ Hordeum vulgare
2.7.7.44 Co2+ 40% of the activation with Mg2+ Hordeum vulgare
2.7.7.44 Mg2+ best fulfills the requirement for a divalent cation, maximal activity of forward reaction at a Mg2+/UTP ratio of 2:1. In the reverse reaction below 5 mM diphosphate, a Mg2+:diphosphate ratio of approximately 1:1 gives optimal activity, excess of Mg2+ is inhibitory Hordeum vulgare
2.7.7.44 Mn2+ can substitute for Mg2+ in either the forward or the reverse direction, 53% of the activation with Mg2+, excess of Mn2+ inhibits the reaction in both directions Hordeum vulgare
2.7.7.44 Zn2+ 37% of the activation with Mg2+ Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.44 UTP + D-glucuronic acid 1-phosphate Hordeum vulgare predominant route whereby UDPglucuronic acid is termed in young barley seedlings ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.44 Hordeum vulgare
-
 var. Larker
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.44 partial Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.44 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.44 3.8
-
-
 Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.7.7.44 -20°C, if dialyzed and stored deep frozen the enzyme rapidly loses activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.44 UTP + D-glucuronic acid 1-phosphate
-
 Hordeum vulgare diphosphate + UDP-D-glucuronic acid
-
 r
2.7.7.44 UTP + D-glucuronic acid 1-phosphate at 16% of the activity observed with UTP Hordeum vulgare diphosphate + UDP-D-glucuronic acid
-
 r
2.7.7.44 UTP + D-glucuronic acid 1-phosphate predominant route whereby UDPglucuronic acid is termed in young barley seedlings Hordeum vulgare ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.44 8 9
-
 Hordeum vulgare