EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.8.7 | biotechnology | mutant ACPs will be valuable in dissecting the structure-function relationships of ACP and its participation in synthesis and regulation of fatty acid, phospholipids, and other products of medical and biotechnological importance | Vibrio harveyi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.7 | acpP gene amplified from a genomic Vibrio harveyi library by PCR, cloned and transformed in to Escherichia coli Bl-21 | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 0.003 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, mutant acyl carrier protein Y71A | Vibrio harveyi | |
2.7.8.7 | 0.0073 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, mutant acyl carrier protein V12G | Vibrio harveyi | |
2.7.8.7 | 0.0079 | - |
apo-acyl-carrier protein | pH 7.8, 37°C | Vibrio harveyi | |
2.7.8.7 | 0.0087 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, r acyl carrier protein | Vibrio harveyi | |
2.7.8.7 | 0.0094 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, Escherichia coli acyl carrier protein | Vibrio harveyi | |
2.7.8.7 | 0.013 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, mutant acyl carrier protein A59G | Vibrio harveyi | |
2.7.8.7 | 0.017 | - |
apo-ACP | pH 7.8, 37°C, mutant acyl carrier protein F50A | Vibrio harveyi | |
2.7.8.7 | 0.029 | - |
apo-acyl-carrier protein | pH 7.8, 37°C, mutant acyl carrier protein I54L | Vibrio harveyi | |
2.7.8.7 | 0.035 | - |
apo-ACP | pH 7.8, 37°C, mutant ACP I54V | Vibrio harveyi | |
6.2.1.20 | additional information | - |
additional information | Km values given for several mutant acyl-carrier proteins | Vibrio harveyi | |
6.2.1.20 | 7.9 | - |
acyl-carrier protein | pH 7.8, 37°C | Vibrio harveyi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.7 | Mg2+ | - |
Vibrio harveyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.7 | CoA + apo-[acyl-carrier protein] | Vibrio harveyi | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
6.2.1.20 | ATP + an acid + [acyl-carrier protein] | Vibrio harveyi | synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.7 | Vibrio harveyi | P0A2W3 | acpP gene | - |
6.2.1.20 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.20 | partial | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.7 | CoA + apo-[acyl-carrier protein] | - |
Vibrio harveyi | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
2.7.8.7 | additional information | Escherichia coli ACP and mutant ACP proteins rACP, V12G, F50A, I54L, I154V, A59G and Y71A are substrates, mutant I54A is no substrate | Vibrio harveyi | ? | - |
? | |
6.2.1.20 | ATP + an acid + [acyl-carrier protein] | synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives | Vibrio harveyi | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? | |
6.2.1.20 | ATP + an acid + [acyl-carrier protein] | mutations in acyl-carrier protein F50A, I54L and I54V increases Km-values, minor effects with mutations at positions V12G, Y71A and A59G | Vibrio harveyi | AMP + diphosphate + acyl-[acyl-carrier protein] | - |
? |