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Literature summary extracted from

  • Flaman, A.S.; Chen, J.M.; Van Iderstine, S.C.; Byers, D.M.
    Site-directed mutagenesis of acyl carrier protein (ACP) reveals amino acid residues involved in ACP structure and acyl-ACP synthetase activity (2001), J. Biol. Chem., 276, 35934-35939.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.8.7 biotechnology mutant ACPs will be valuable in dissecting the structure-function relationships of ACP and its participation in synthesis and regulation of fatty acid, phospholipids, and other products of medical and biotechnological importance Vibrio harveyi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 acpP gene amplified from a genomic Vibrio harveyi library by PCR, cloned and transformed in to Escherichia coli Bl-21 Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.003
-
apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein Y71A Vibrio harveyi
2.7.8.7 0.0073
-
apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein V12G Vibrio harveyi
2.7.8.7 0.0079
-
apo-acyl-carrier protein pH 7.8, 37°C Vibrio harveyi
2.7.8.7 0.0087
-
apo-acyl-carrier protein pH 7.8, 37°C, r acyl carrier protein Vibrio harveyi
2.7.8.7 0.0094
-
apo-acyl-carrier protein pH 7.8, 37°C, Escherichia coli acyl carrier protein Vibrio harveyi
2.7.8.7 0.013
-
apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein A59G Vibrio harveyi
2.7.8.7 0.017
-
apo-ACP pH 7.8, 37°C, mutant acyl carrier protein F50A Vibrio harveyi
2.7.8.7 0.029
-
apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein I54L Vibrio harveyi
2.7.8.7 0.035
-
apo-ACP pH 7.8, 37°C, mutant ACP I54V Vibrio harveyi
6.2.1.20 additional information
-
additional information Km values given for several mutant acyl-carrier proteins Vibrio harveyi
6.2.1.20 7.9
-
acyl-carrier protein pH 7.8, 37°C Vibrio harveyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+
-
Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Vibrio harveyi
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
6.2.1.20 ATP + an acid + [acyl-carrier protein] Vibrio harveyi synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives AMP + diphosphate + acyl-[acyl-carrier protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Vibrio harveyi P0A2W3 acpP gene
-
6.2.1.20 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 partial Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
Vibrio harveyi adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
2.7.8.7 additional information Escherichia coli ACP and mutant ACP proteins rACP, V12G, F50A, I54L, I154V, A59G and Y71A are substrates, mutant I54A is no substrate Vibrio harveyi ?
-
?
6.2.1.20 ATP + an acid + [acyl-carrier protein] synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates and donor for activated acyl groups for the synthesis of lipids and lipid derivatives Vibrio harveyi AMP + diphosphate + acyl-[acyl-carrier protein]
-
?
6.2.1.20 ATP + an acid + [acyl-carrier protein] mutations in acyl-carrier protein F50A, I54L and I54V increases Km-values, minor effects with mutations at positions V12G, Y71A and A59G Vibrio harveyi AMP + diphosphate + acyl-[acyl-carrier protein]
-
?