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Literature summary extracted from

  • Hatch, M.D.; Slack, C.R.
    Pyruvate, Pi dikinase from leaves (1975), Methods Enzymol., 42C, 212-219.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.9.1 thiols required for activity in solution Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 ADP mediates a rapid but reversible inactivation in presence of a thiol Arundinaria sp.
2.7.9.1 ADP mediates a rapid but reversible inactivation in presence of a thiol Zea mays
2.7.9.1 AMP
-
 Arundinaria sp.
2.7.9.1 AMP
-
 Zea mays
2.7.9.1 gamma(p-Arsenophenyl)-n-butyrate
-
 Arundinaria sp.
2.7.9.1 gamma(p-Arsenophenyl)-n-butyrate
-
 Zea mays
2.7.9.1 p-chloromercuribenzoate
-
 Arundinaria sp.
2.7.9.1 p-chloromercuribenzoate
-
 Zea mays
2.7.9.1 p-hydroxymercuribenzoate
-
 Zea mays
2.7.9.1 phosphate
-
 Arundinaria sp.
2.7.9.1 phosphate
-
 Zea mays
2.7.9.1 pyruvate
-
 Arundinaria sp.
2.7.9.1 pyruvate
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.004
-
 AMP pH 6.8, 25ºC Arundinaria sp.
2.7.9.1 0.009
-
 ATP pH 8.3, 30ºC Zea mays
2.7.9.1 0.04
-
 diphosphate
-
 Zea mays
2.7.9.1 0.04
-
 diphosphate
-
 Arundinaria sp.
2.7.9.1 0.09
-
 ATP pH 8.3 Zea mays
2.7.9.1 0.11
-
 pyruvate pH 8.3, 30ºC Zea mays
2.7.9.1 0.11
-
 pyruvate pH 8.3, 30ºC Arundinaria sp.
2.7.9.1 0.5
-
 phosphate pH 8.3, 30ºC Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Mg2+
-
 Zea mays
2.7.9.1 Mn2+ not activation Zea mays
2.7.9.1 NH4+
-
 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 94000
-
 4 * 94000, SDS-PAGE Zea mays
2.7.9.1 387000
-
 sedimentation analysis Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Arundinaria sp.
-
-
-
2.7.9.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 partial, using Sephadex-G200 and Hypatite C chromatography Zea mays

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate Zea mays
a
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate Arundinaria sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 leaf
-
 Zea mays
-
2.7.9.1 leaf
-
 Arundinaria sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 1.2
-
 measured at 30ºC Zea mays

Storage Stability

EC Number Storage Stability Organism
2.7.9.1 0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 Zea mays
2.7.9.1 0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 Arundinaria sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + arsenate
-
 Zea mays ?
-
 ir
2.7.9.1 ATP + pyruvate + arsenate
-
 Arundinaria sp. ?
-
 ir
2.7.9.1 ATP + pyruvate + phosphate
-
 Arundinaria sp. AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate activity with UTP, GTP and CTP is 1-3% of the activity with ATP Zea mays AMP + phosphoenolpyruvate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 tetramer 4 * 94000, SDS-PAGE Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.9.1
-
-
 rapid inactivation, activity is recovered by re-warming to 20-30ºC Zea mays
2.7.9.1
-
-
 rapid inactivation, activity is recovered by re-warming to 20-30ºC Arundinaria sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.1 8.3
-
 phosphoenolpyruvate formation Arundinaria sp.