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Literature summary extracted from

  • Sugiyama, T.
    Purification, molecular, and catalytic properties of pyruvate phosphate dikinase from the maize leaf (1973), Biochemistry, 12, 2862-2868.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.9.1 method description Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.015
-
 ATP pH 7.5, 22ºC, phosphoenolpyruvate formation Zea mays
2.7.9.1 0.04
-
 diphosphate pH 7.5, 22ºC, pyruvate formation Zea mays
2.7.9.1 0.14
-
 phosphoenolpyruvate pH 7.5, 22ºC, pyruvate formation Zea mays
2.7.9.1 0.25
-
 pyruvate pH 7.5, 22ºC, phosphoenolpyruvate formation Zea mays
2.7.9.1 1.5
-
 phosphate pH 7.5, 22ºC, phosphoenolpyruvate formation Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Mg2+ free Mg2+ is required to stabilize the oligomeric structure of the enzyme Zea mays
2.7.9.1 NH4+ at saturated concentration of NH4Cl pyruvate formation activity is increased 20fold Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 94000
-
 4 * 94000, SDS-PAGE Zea mays
2.7.9.1 370000
-
 gel filtration on Sepharose 6B Zea mays
2.7.9.1 387000
-
 sedimentation analysis Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 additional information Zea mays involved in C4 dicarboxylic acid pathway in plant ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 leaf
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 4.5
-
-
 Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Zea mays AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 additional information involved in C4 dicarboxylic acid pathway in plant Zea mays ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 tetramer 4 * 94000, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.1 22
-
 assay at Zea mays