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Literature summary extracted from

  • Magahara, N.; Nishino, T.
    Role of amino acid residues in the active site of rat liver mercaptopyruvate sulfurtransferase. CDNA cloning, overexpression, and site-directed mutagenesis (1996), J. Biol. Chem., 271, 27395-27401.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.2
-
 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.2 C247S no enzyme activity Rattus norvegicus
2.8.1.2 C248R slightly increased Km Rattus norvegicus
2.8.1.2 R178G increased Km Rattus norvegicus
2.8.1.2 R196G increased Km Rattus norvegicus
2.8.1.2 R248G facilitated catalysis of thiosulfate Rattus norvegicus
2.8.1.2 S249A no significant difference in Km compared to wild-type enzyme Rattus norvegicus
2.8.1.2 S249K facilitated catalysis of thiosulfate Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.2 0.49
-
 3-Mercaptopyruvate S249A mutant Rattus norvegicus
2.8.1.2 1.1
-
 3-Mercaptopyruvate S249K mutant Rattus norvegicus
2.8.1.2 1.2
-
 3-Mercaptopyruvate wild-type enzyme Rattus norvegicus
2.8.1.2 2.1
-
 3-Mercaptopyruvate G248R mutant Rattus norvegicus
2.8.1.2 11
-
 3-Mercaptopyruvate R196G mutant Rattus norvegicus
2.8.1.2 70
-
 3-Mercaptopyruvate R187G mutant Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide Rattus norvegicus
-
 pyruvate + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.2 Rattus norvegicus P97532
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.2
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.2 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Rattus norvegicus pyruvate + thiocyanate
-
 ?