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Literature summary extracted from

  • Chauncey, T.R.; Westley, J.
    Improved purification and sulfhydryl analysis of thiosulfate reductase (1983), Biochim. Biophys. Acta, 744, 304-311.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.1.3 extremely labile during all ion-exchange steps Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.3 additional information alkylation of cysteine residues with iodoacetate or iodoacetamide does not inactivate Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.3 thiosulfate + glutathione Saccharomyces cerevisiae
-
 sulfite + glutathione disulfide + sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.3 Saccharomyces cerevisiae
-
 Red Star brand
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.3 900fold Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.3 thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.3 additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.8.1.3 -40°C, 50 mM Tris-acetate, pH 8.0, 0.5 mM Na2S2O3, 50% glycerol, stable for at least 9 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.3 thiosulfate + glutathione
-
 Saccharomyces cerevisiae sulfite + glutathione disulfide + sulfide
-
 ?
2.8.1.3 thiosulfate + glutathione
-
 Saccharomyces cerevisiae sulfite + glutathione disulfide + sulfide the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide ?