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Literature summary extracted from

  • Schmidt, A.; Erdle, I.; Gamon, B.
    Isolation and characterization of thiosulfate reductases from the green alga Chlorella fusca (1984), Planta, 162, 243-249.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.5 thiosulfate above 0.5 mM [Chlorella] fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.5 0.156
-
thiosulfate pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR II [Chlorella] fusca
2.8.1.5 0.164
-
thiosulfate pH 9, 37°C, with dithioerythritol as second substrate, isoenzyme TSR I [Chlorella] fusca
2.8.1.5 1.54
-
dithioerythritol pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR II [Chlorella] fusca
2.8.1.5 3.1
-
dithioerythritol pH 9, 37°C, with thiosulfate as second substrate, isoenzyme TSR I [Chlorella] fusca
2.8.1.5 20
-
KCN pH 9, 37°C, with dithioerythritol as second substrate [Chlorella] fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.5 24000
-
isoenzyme IIIb, gel filtration [Chlorella] fusca
2.8.1.5 26500
-
isoenzyme II, gel filtration [Chlorella] fusca
2.8.1.5 28000
-
isoenzyme I, gel filtration [Chlorella] fusca
2.8.1.5 55000
-
isoenzyme IIIa, gel filtration [Chlorella] fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.5 thiosulfate + 2 dithioerythritol [Chlorella] fusca
-
sulfite + dithioerythritol disulfide + sulfide
-
?
2.8.1.5 thiosulfate + 2 dithioerythritol [Chlorella] fusca 211-8b
-
sulfite + dithioerythritol disulfide + sulfide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.5 [Chlorella] fusca
-
-
-
2.8.1.5 [Chlorella] fusca 211-8b
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.5 isoenzymes: TSR I, TSR II, TSR IIIa, TSR IIIb [Chlorella] fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.5 additional information
-
-
[Chlorella] fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.5 cyanate + dithioerythritol activity only in presence of thiols [Chlorella] fusca ?
-
?
2.8.1.5 cyanate + dithioerythritol activity only in presence of thiols [Chlorella] fusca 211-8b ?
-
?
2.8.1.5 KCN + dithioerythritol
-
[Chlorella] fusca ?
-
?
2.8.1.5 KCN + dithioerythritol
-
[Chlorella] fusca 211-8b ?
-
?
2.8.1.5 additional information little activity with: glutathione, L-cysteine, beta-mercaptoethanol [Chlorella] fusca ?
-
?
2.8.1.5 additional information little activity with: glutathione, L-cysteine, beta-mercaptoethanol [Chlorella] fusca 211-8b ?
-
?
2.8.1.5 thiosulfate + 2 dithioerythritol
-
[Chlorella] fusca sulfite + dithioerythritol disulfide + sulfide
-
?
2.8.1.5 thiosulfate + 2 dithioerythritol
-
[Chlorella] fusca 211-8b sulfite + dithioerythritol disulfide + sulfide
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.5 37
-
assay at [Chlorella] fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.5 8.5
-
isoenzyme TSR IIIa [Chlorella] fusca
2.8.1.5 9
-
isoenzyme TSRI and TSR II [Chlorella] fusca
2.8.1.5 9.5
-
isoenzyme TSR IIIb [Chlorella] fusca

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.5 thioredoxin stimulates thiosulfate reductase IIIa and IIIb [Chlorella] fusca