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Literature summary extracted from

  • Kim, D.H.; Konishi, L.; Kobashi, K.
    Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine (1986), Biochim. Biophys. Acta, 872, 33-41.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.2.22 4-nitrophenyl sulfate, stabilizes against thermal inactivation and against inactivation on storage under cold conditions Eubacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.22 4-chloromercuribenzenesulfonic acid 50% inhibition at 0.0000038 mM Eubacterium sp.
2.8.2.22 Cu2+ 50% inhibition at 0.01 mM Eubacterium sp.
2.8.2.22 diethyl dicarbonate 50% inhibition at 0.28 mM Eubacterium sp.
2.8.2.22 DTNB 50% inhibition at 0.023 mM Eubacterium sp.
2.8.2.22 EDTA inactivation reversed by addition of metal ions Eubacterium sp.
2.8.2.22 Fe2+ 50% inhibition at 0.6 mM Eubacterium sp.
2.8.2.22 Hg2+ 50% inhibition at 0.001 mM Eubacterium sp.
2.8.2.22 N-tosyl-L-lysylchloromethane 50% inhibition at 0.04 mM Eubacterium sp.
2.8.2.22 Ni2+ 50% inhibition at 1 mM Eubacterium sp.
2.8.2.22 PCMB 50% inhibition at 0.000025 mM Eubacterium sp.
2.8.2.22 Zn2+ 50% inhibition at 0.2 mM Eubacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.22 0.104
-
 4-nitrophenyl sulfate with tyramine as second substrate, pH 8, 37°C Eubacterium sp.
2.8.2.22 3.5
-
 tyramine with 4-nitrophenyl sulfate as second substrate, pH 8, 37°C Eubacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.22 Mg2+ increases activity by 10-20% Eubacterium sp.
2.8.2.22 Mn2+ increases activity by 10-20% Eubacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 80000
-
 4 * 80000 SDS-PAGE Eubacterium sp.
2.8.2.22 315000
-
 gel filtration Eubacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Eubacterium sp.
-
 A-44
-
2.8.2.22 Eubacterium sp. A-44
-
 A-44
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22
-
 Eubacterium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.22 an aryl sulfate + a phenol = a phenol + an aryl sulfate mechanism Eubacterium sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.22 89.4
-
-
 Eubacterium sp.

Storage Stability

EC Number Storage Stability Organism
2.8.2.22 4°C or -20°C, more than 50% loss of activity after 1 month Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Eubacterium sp. phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenol
-
 Eubacterium sp. A-44 phenyl sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenolphthalein
-
 Eubacterium sp. phenolphthalein O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + phenolphthalein
-
 Eubacterium sp. A-44 phenolphthalein O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + salicylamide
-
 Eubacterium sp. salicylamide O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + salicylamide
-
 Eubacterium sp. A-44 salicylamide O-sulfate + 4-nitrophenol
-
 ?
2.8.2.22 4-nitrophenyl sulfate + tyramine
-
 Eubacterium sp. 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol i.e. tyramine O-sulfate ?
2.8.2.22 4-nitrophenyl sulfate + tyramine
-
 Eubacterium sp. A-44 4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol i.e. tyramine O-sulfate ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 tetramer 4 * 80000 SDS-PAGE Eubacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.22 37
-
 assay at Eubacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.22 45
-
 10 min, pH 5.5-7, 50% loss of activity Eubacterium sp.
2.8.2.22 55
-
 10 min, pH 5.5-7, complete loss of activity Eubacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.22 8
-
 tyramine, salicylamide Eubacterium sp.
2.8.2.22 8.5
-
 phenol Eubacterium sp.
2.8.2.22 9
-
 phenolphthalein Eubacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.8.2.22 5.5 7 most stable pH for storage Eubacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.22 Eubacterium sp.
-
-
 3.9