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Literature summary extracted from

  • Fenselau, A.; Wallis, K.
    Substrate specificity and mechanism of action of acetoacetate coenzyme A transferase from rat heart (1974), Biochemistry, 13, 3884-3889.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.5 2,2-Difluorosuccinate strong Rattus norvegicus
2.8.3.5 3-Sulfopropanoate
-
 Rattus norvegicus
2.8.3.5 acetoacetate
-
 Rattus norvegicus
2.8.3.5 acetylene dicarboxylate weak Rattus norvegicus
2.8.3.5 Maleamate reversible, competitive Rattus norvegicus
2.8.3.5 Maleimide succinate or acetoacetate protects Rattus norvegicus
2.8.3.5 malonate kinetics Rattus norvegicus
2.8.3.5 Monomethylsuccinate competitive Rattus norvegicus
2.8.3.5 additional information no inhibition by glutarate, adipate, cis- or trans-cyclobutane-1,2-dicarboxylate, cis- or trans-cyclohexane-1,2-dicarboxylate, methylsuccinate, mercaptosuccinate, malate, aspartate, succinimide or iodoacetamide Rattus norvegicus
2.8.3.5 N-ethylmaleamate reversible, competitive Rattus norvegicus
2.8.3.5 N-ethylmaleimide succinate or acetoacetate protects Rattus norvegicus
2.8.3.5 oxalate kinetics Rattus norvegicus
2.8.3.5 Perfluorosuccinate strong Rattus norvegicus
2.8.3.5 succinamate competitive Rattus norvegicus
2.8.3.5 succinate product inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.5 0.04
-
 acetoacetyl-CoA pH 8.1, 25°C Rattus norvegicus
2.8.3.5 0.28
-
 succinyl-CoA pH 8.1, 25°C Rattus norvegicus
2.8.3.5 0.44
-
 acetoacetate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 28
-
 succinate cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C Rattus norvegicus
2.8.3.5 35
-
 Maleate cosusbstrate: acetoacetyl-CoA, pH 8.1, 25°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.5 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Rattus norvegicus
-
 male adult Buffalo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.5 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.5 heart
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.5 2.88
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.8.3.5 frozen, partially purified preparation in phosphate solution, less than 10% loss of activity within 1-2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 additional information no substrates are diacids with connecting chain lengths of 3 or more methylene groups Rattus norvegicus ?
-
 ?
2.8.3.5 succinyl-CoA + acetoacetate
-
 Rattus norvegicus succinate + acetoacetyl-CoA
-
 r
2.8.3.5 succinyl-CoA + maleate
-
 Rattus norvegicus succinate + maleyl-CoA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.5 25
-
 assay at Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.5 0.72
-
 succinate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 3.7
-
 acetoacetate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 6.4
-
 2,2-Difluorosuccinate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 15
-
 oxalate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 18
-
 Perfluorosuccinate pH 8.1, 25°C Rattus norvegicus
2.8.3.5 21
-
 malonate pH 8.1, 25°C Rattus norvegicus