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Literature summary extracted from

  • Gobel, M.; Kassel-Cati, K.; Schmidt, E.; Reineke, W.
    Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase (2002), J. Bacteriol., 184, 216-223.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.174
-
 Pseudomonas sp.
2.8.3.6 pcaI and pcaJ-genes encoding the two subunits of the enzyme Pseudomonas knackmussii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.174 41678
-
 x * 41678, calculation from nucleotide sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.174 CoA + 3-oxoadipyl-CoA Pseudomonas sp. 3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates succinyl-CoA + acetyl-CoA
-
 ?
2.3.1.174 CoA + 3-oxoadipyl-CoA Pseudomonas sp. B13 3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates succinyl-CoA + acetyl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 3-oxoadipate Pseudomonas knackmussii penultimate step in conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates utilizing the beta-ketoadipate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.174 Pseudomonas sp.
-
-
-
2.3.1.174 Pseudomonas sp. B13
-
-
-
2.8.3.6 Pseudomonas knackmussii Q8VPF3 and Q8VPF2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.6 4.6
-
 crude extract Pseudomonas knackmussii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.174 CoA + 3-oxoadipyl-CoA 3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates Pseudomonas sp. succinyl-CoA + acetyl-CoA
-
 ?
2.3.1.174 CoA + 3-oxoadipyl-CoA 3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates Pseudomonas sp. B13 succinyl-CoA + acetyl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 3-oxoadipate penultimate step in conversion of benzoate and 4-hydroxybenzoate to tricarboxylic acid cycle intermediates utilizing the beta-ketoadipate pathway Pseudomonas knackmussii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.174 ? x * 41678, calculation from nucleotide sequence Pseudomonas sp.