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Literature summary extracted from

  • Alexson, S.E.H.; Svensson, L.T.; Nedergaard, J.
    NADH-sensitive propionyl-CoA hydrolase in brown adipose tissue mitochondria of the rat (1989), Biochim. Biophys. Acta, 1005, 13-19.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 additional information cold acclimation induces enzyme activity Rattus norvegicus
3.1.2.18 additional information cold acclimation induces enzyme activity Rattus norvegicus
3.1.2.19 additional information cold acclimation induces enzyme activity Rattus norvegicus
3.1.2.20 additional information cold acclimation induces enzyme activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 NADH no inhibition Rattus norvegicus
3.1.2.18 beta-NADH 91% inhibition at 0.4 mM; regulatory function Rattus norvegicus
3.1.2.19 beta-NADH weak inhibition, 46% at 0.4 mM Rattus norvegicus
3.1.2.20 NADH no inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.2 mitochondrion matrix Rattus norvegicus 5739
-
3.1.2.18 mitochondrial matrix
-
 Rattus norvegicus 5759
-
3.1.2.18 mitochondrion
-
 Rattus norvegicus 5739
-
3.1.2.18 additional information submitochondrial and subcellular distribution Rattus norvegicus
-
-
3.1.2.19 mitochondrial matrix
-
 Rattus norvegicus 5759
-
3.1.2.19 mitochondrion
-
 Rattus norvegicus 5739
-
3.1.2.19 additional information submitochondrial and subcellular distribution Rattus norvegicus
-
-
3.1.2.20 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.20 mitochondrion matrix Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 100000
-
 gel filtration Rattus norvegicus
3.1.2.2 100000
-
 below Rattus norvegicus
3.1.2.18 240000
-
 at least, gel filtration Rattus norvegicus
3.1.2.19 100000
-
 about, gel filtration Rattus norvegicus
3.1.2.20 100000
-
 gel filtration Rattus norvegicus
3.1.2.20 100000
-
 below Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.18 acyl-CoA + H2O Rattus norvegicus
-
 CoA + a carboxylate
-
 ?
3.1.2.18 propionyl-CoA + H2O Rattus norvegicus ensures that the propionyl-CoA level in mitochondria is not detrimentally increased CoA + propionate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.18 Rattus norvegicus
-
 adult femal Sprague-Dawley rats
-
3.1.2.19 Rattus norvegicus
-
 adult female Sprague-Dawley rats
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 partial Rattus norvegicus
3.1.2.18 partial Rattus norvegicus
3.1.2.19 partial Rattus norvegicus
3.1.2.20 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 brown adipose tissue
-
 Rattus norvegicus
-
3.1.2.18 brown adipose tissue
-
 Rattus norvegicus
-
3.1.2.19 brown adipose tissue
-
 Rattus norvegicus
-
3.1.2.20 brown adipose tissue
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 0.003 0.009 partially purified enzyme, medium- and long chain fatty acid hydrolase activity Rattus norvegicus
3.1.2.20 0.003 0.009 partially purified enzyme, medium- and long chain fatty acid hydrolase activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.18 acetyl-CoA + H2O low activity Rattus norvegicus acetate + CoA
-
 ?
3.1.2.18 acyl-CoA + H2O
-
 Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.18 propionyl-CoA + H2O best substrate Rattus norvegicus CoA + propionate
-
 ?
3.1.2.18 propionyl-CoA + H2O ensures that the propionyl-CoA level in mitochondria is not detrimentally increased Rattus norvegicus CoA + propionate
-
 ?
3.1.2.19 additional information broad substrate spectrum, substrate specificity Rattus norvegicus ?
-
 ?
3.1.2.19 additional information from the substrate specificity curves it is not possible to conclude whether the long-chain acyl-CoA hydrolase activity is due to the broad-spectrum enzyme or to a different long-chain acyl-CoA hydrolase Rattus norvegicus ?
-
 ?
3.1.2.19 nonanoyl-CoA + H2O best substrate Rattus norvegicus CoA + nonanoate
-
 ?
3.1.2.20 acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.4
-
 assay at Rattus norvegicus
3.1.2.18 7.4
-
 assay at Rattus norvegicus
3.1.2.20 7.4
-
 assay at Rattus norvegicus